Parkin Antikörper (pSer378)

Produktnummer ABIN361515

Dieses Anti-Parkin-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Parkin in WB. Geeignet für Human.

Kurzübersicht für Parkin Antikörper (pSer378) (ABIN361515)

Target: Parkin (PARK2) (Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Parkin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Parkin Antikörper

Bindungsspezifität: pSer378

Spezifität: Specific for the ~52k parkin protein phosphorylated at Ser378. Immunolabeling of the parkin band is absent in parkin S378 mutants.

Kreuzreaktivität: Human

Aufreinigung: Antigen Affinity Purified

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser378 conjugated to KLH

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Lagerung: -20 °C

Details zu Parkin

Target: Parkin (PARK2) (Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2))

Andere Bezeichnung: PARK2

Hintergrund: Parkin is an E3 ligase in the ubiquitin-proteasome system. Hereditary Parkinson’s disease is most commonly caused by mutations in the parkin gene and is characterized by the progressive loss of dopaminergic neurons and the presence of Lewy bodies in the substania nigra (Jenner et al.,1992). Recent evidence suggests that phosphorylation of parkin at Ser378 may have an important regulatory role on its E3 ubiquitin ligase activity (Yamamoto et al., 2005). Anti-Phospho-Ser378 Parkin Western blot of HEK293 cells transfected with Parkin WT (Phospho) and Parkin S378 mutant (non-phospho) showing the phospho-specific immunolabeling of the ~ 52 k parkin protein. The immunolabeling is absent in the parkin S378 mutant.

Molekulargewicht: '52 kDa

Gen-ID: 5071

UniProt: O60260

Pathways: Autophagie, Ubiquitin Proteasome Pathway