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AKT1 Antikörper (pSer473)

Dieses Anti-AKT1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von AKT1 in WB, ELISA, IHC, IF, FACS, IP, FM und MA. Geeignet für Human, Maus, Ratte und Affe. Dieses Primary Antibody wurde in 3+ Publikationen zitiert.
Rockland
Produktnummer ABIN349614
Hersteller Produkt- Nr.: 200-301-268

Kurzübersicht für AKT1 Antikörper (pSer473) (ABIN349614)

Target

Alle AKT1 Antikörper anzeigen
AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Reaktivität

  • 488
  • 312
  • 248
  • 54
  • 51
  • 45
  • 33
  • 17
  • 12
  • 11
  • 5
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Human, Maus, Ratte, Affe

Wirt

  • 406
  • 103
  • 7
  • 2
  • 1
  • 1
Maus

Klonalität

  • 360
  • 159
Monoklonal

Konjugat

  • 305
  • 29
  • 20
  • 20
  • 19
  • 13
  • 10
  • 10
  • 8
  • 8
  • 8
  • 8
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
Dieser AKT1 Antikörper ist unkonjugiert

Applikation

  • 405
  • 233
  • 171
  • 89
  • 77
  • 69
  • 59
  • 50
  • 38
  • 35
  • 31
  • 18
  • 15
  • 9
  • 9
  • 8
  • 6
  • 4
  • 3
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Fluorescence Microscopy (FM), Multiplex Assay (MA)

Klon

17F6-B11
  • Bindungsspezifität

    • 88
    • 44
    • 35
    • 33
    • 28
    • 23
    • 19
    • 18
    • 16
    • 12
    • 11
    • 10
    • 9
    • 8
    • 8
    • 8
    • 8
    • 7
    • 6
    • 6
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    pSer473

    Hersteller Produkt- Nr.

    200-301-268

    Hersteller

    Rockland

    Verwendungszweck

    AKT phospho S473 Antibody

    Kreuzreaktivität (Details)

    This phospho specific monoclonal antibody is specific for phosphorylated human and mouse AKT protein at S473.

    Produktmerkmale

    Synonyms: mouse anti-AKT pS473 Antibody, phospho AKT, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1

    Aufreinigung

    Anti-AKT pS473 Monoclonal Antibody was purified from concentrated tissue culture supernate by Protein A chromatography.

    Sterilität

    Sterile filtered

    Immunogen

    Immunogen: Anti-AKT pS473 (MOUSE) Monoclonal Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein, followed by hybridoma development.

    Immunogen Type: Conjugated Peptide

    Isotyp

    IgG1 kappa
  • Applikationshinweise

    Flow Cytometry Dilution: User Optimized

    Immunohistochemistry Dilution: 20 μg/mL

    Application Note: Phospho AKT antibody is tested in ELISA, immunofluorescence, immunohistochemistry, flow cytometry, and western blotting. Expect a band approximately 56 kDa in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pS473 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry use formalin-fixed paraffin-embedded sections. No pre-treatment of sample is required.

    Western Blot Dilution: 1:500-1:3,000

    Immunoprecipitation Dilution: User Optimized

    ELISA Dilution: 1:20,000

    IF Microscopy Dilution: 1:500-1:3,000

    Other: User Optimized

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    1.02 mg/mL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store Anti-AKT pS473 (MOUSE) Monoclonal Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    Haltbarkeit

    12 months
  • Maerz, Burkhalter, Schilpp, Wittekindt, Frick, Philipp: "Pharmacological cholesterol depletion disturbs ciliogenesis and ciliary function in developing zebrafish." in: Communications biology, Vol. 2, pp. 31, (2019) (PubMed).

    Sha, Thompson, South, Baron, Leask: "Loss of PPARγ expression by fibroblasts enhances dermal wound closure." in: Fibrogenesis & tissue repair, Vol. 5, pp. 5, (2012) (PubMed).

    Ito, Nishibori, Ito, Takagi, Akimoto, Kudo, Asanuma, Sai, Miyamoto, Takenaka, Yan: "mTORC1 activation triggers the unfolded protein response in podocytes and leads to nephrotic syndrome." in: Laboratory investigation; a journal of technical methods and pathology, (2011) (PubMed).

  • Target

    AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

    Andere Bezeichnung

    AKT1

    Hintergrund

    Background: AKT phospho S473 is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation. Anti-AKT pS473 (MOUSE) Monoclonal Antibody is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience, Signal Transduction research.

    Gen-ID

    207, 62241011

    UniProt

    P31749

    Pathways

    PI3K-Akt Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, AMPK Signaling, Interferon-gamma Pathway, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, Warburg Effekt
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