CD14 Antikörper (AA 9-13, AA 39-44)

Produktnummer ABIN343168

Dieses Maus Monoklonal-Antikörper erkennt spezifisch CD14 in FACS, WB, ELISA und Agon. Er zeigt eine Reaktivität gegenüber Human und wurde in 5+ Publikationen erwähnt.

Kurzübersicht für CD14 Antikörper (AA 9-13, AA 39-44) (ABIN343168)

Target: CD14

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD14 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), ELISA, Agonist (Agon)

Klon: BiG13

Produktdetails anti-CD14 Antikörper

Bindungsspezifität: AA 9-13, AA 39-44

Spezifität: Soluble and membrane bound human CD14. The antibody blocks LPS binding to membrane bound CD14

Kreuzreaktivität: Corynebacterium diphtheriae, Rind (Kuh), Hund, Pferd, Schwein

Kreuzreaktivität (Details): CD14 of cattle++, swine ++, dog+ horse+

Aufreinigung: Protein G affinity column

Immunogen: Monocytes of different species and immunoaffinity purified soluble human CD14

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: 20 μg/mL inhibits binding of 0.5 μg/ ml LPS to CD14.Binding titre at human CD14 transfected CHO-cells (FACS): more than 100.000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with bidistilled water

Konzentration: 1 mg/mL

Buffer: PBS, pH 7.5

Konservierungsmittel: Without preservative

Lagerung: -20 °C,-80 °C

Referenzen für anti-CD14 Antikörper (ABIN343168)

Moyo, Marchi, Steinbach: "Differentiation and activation of equine monocyte-derived dendritic cells are not correlated with CD206 or CD83 expression." in: Immunology, Vol. 139, Issue 4, pp. 472-83, (2013) (PubMed).

Bache, Spreitzer, Becker, Loeschner, Rosskopf, Hanschmann, Schwanig, Schneider, Lieb, Montag: "Bordetella Pertussis Toxin does not induce the release of pro-inflammatory cytokines in human whole blood." in: Medical microbiology and immunology, Vol. 201, Issue 3, pp. 327-35, (2012) (PubMed).

Kato, Ogasawara, Homma, Saito, Matsumoto: "Lipopolysaccharide-binding protein critically regulates lipopolysaccharide-induced IFN-beta signaling pathway in human monocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 10, pp. 6185-94, (2004) (PubMed).

Berner, Fürll, Stelter, Dröse, Müller, Schütt: "Elevated levels of lipopolysaccharide-binding protein and soluble CD14 in plasma in neonatal early-onset sepsis." in: Clinical and diagnostic laboratory immunology, Vol. 9, Issue 2, pp. 440-5, (2002) (PubMed).

Vidal, Labéta, Schiffrin, Donnet-Hughes: "Soluble CD14 in human breast milk and its role in innate immune responses." in: Acta odontologica Scandinavica, Vol. 59, Issue 5, pp. 330-4, (2001) (PubMed).

Details zu CD14

Target: CD14

Andere Bezeichnung: CD14

Hintergrund: Background: biG 13 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, weekly at surface of neutrophiles like Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 Molecule has been reported to be a receptor for endotoxin. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for LPS-LBP (lipopolysaccharide binding protein)-complexes.

Molekulargewicht: ~50kDa

Gen-ID: 929

NCBI Accession: NP_000582

UniProt: P08571

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades