Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

CD14 Antikörper

CD14 Reaktivität: Human, Non-Human Primate FACS, WB, ELISA, IP Wirt: Maus Monoclonal MEM-18 unconjugated
Produktnummer ABIN93963
  • Target Alle CD14 Antikörper anzeigen
    CD14
    Reaktivität
    • 268
    • 55
    • 32
    • 30
    • 29
    • 28
    • 25
    • 24
    • 24
    • 18
    • 17
    • 14
    • 10
    • 9
    • 8
    • 8
    • 7
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human, Non-Human Primate
    Wirt
    • 224
    • 64
    • 25
    • 3
    • 1
    Maus
    Klonalität
    • 253
    • 64
    Monoklonal
    Konjugat
    • 128
    • 30
    • 24
    • 18
    • 15
    • 8
    • 6
    • 6
    • 5
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser CD14 Antikörper ist unkonjugiert
    Applikation
    • 223
    • 103
    • 63
    • 58
    • 54
    • 50
    • 22
    • 22
    • 21
    • 14
    • 14
    • 13
    • 7
    • 6
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunoprecipitation (IP)
    Spezifität
    The antibody MEM-18 reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked extracellular membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes, also expressed by most tissue macrophages. In human, the epitope recognized by MEM-18 is located between amino acids 57-64.
    Kreuzreaktivität (Details)
    Non-Human Primates, Human
    Aufreinigung
    Purified by protein-A affinity chromatography.
    Reinheit
    > 95 % (by SDS-PAGE)
    Endotoxin-Niveau
    Low Endotoxin
    Immunogen
    A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.
    Klon
    MEM-18
    Isotyp
    IgG1
    Top Product
    Discover our top product CD14 Primärantikörper
  • Applikationshinweise
    ELISA: The antibody MEM-18 has been tested as the detection antibody in a sandwich ELISA for analysis of human CD14 in combination with antibody B-A8 (cat. no. 11-304-C100).
    Flow cytometry: recommended dilution: 4 μg/mL.
    Western blotting: Non-reducing conditions.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Do not freeze.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store at 2-8°C. Do not freeze.
  • Weiss, Lichtenauer, Kirchner, Stock, Aurich, Christ, Brockhoff, Kunz-Schughart, Jauch, Schlitt, Thasler: "Hepatic progenitor cells from adult human livers for cell transplantation." in: Gut, Vol. 57, Issue 8, pp. 1129-38, (2008) (PubMed).

    Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, (2007) (PubMed).

    Angel, Lala, Chen, Edgar, Ostrovsky, Dunbar: "CD14+ antigen-presenting cells in human dermis are less mature than their CD1a+ counterparts." in: International immunology, Vol. 19, Issue 11, pp. 1271-9, (2007) (PubMed).

    Iwaki, Nishitani, Mitsuzawa, Hyakushima, Sano, Kuroki: "The CD14 region spanning amino acids 57-64 is critical for interaction with the extracellular Toll-like receptor 2 domain." in: Biochemical and biophysical research communications, Vol. 328, Issue 1, pp. 173-6, (2005) (PubMed).

    Sing, Rost, Tvardovskaia, Roggenkamp, Wiedemann, Kirschning, Aepfelbacher, Heesemann: "Yersinia V-antigen exploits toll-like receptor 2 and CD14 for interleukin 10-mediated immunosuppression." in: The Journal of experimental medicine, Vol. 196, Issue 8, pp. 1017-24, (2002) (PubMed).

    Funda, Tucková, Farré, Iwase, Moro, Tlaskalová-Hogenová: "CD14 is expressed and released as soluble CD14 by human intestinal epithelial cells in vitro: lipopolysaccharide activation of epithelial cells revisited." in: Infection and immunity, Vol. 69, Issue 6, pp. 3772-81, (2001) (PubMed).

    Bazil, Baudys, Hilgert, Stefanová, Low, Zbrozek, Horejsí: "Structural relationship between the soluble and membrane-bound forms of human monocyte surface glycoprotein CD14." in: Molecular immunology, Vol. 26, Issue 7, pp. 657-62, (1989) (PubMed).

    Bazil, Horejsí, Baudys, Kristofová, Strominger, Kostka, Hilgert: "Biochemical characterization of a soluble form of the 53-kDa monocyte surface antigen." in: European journal of immunology, Vol. 16, Issue 12, pp. 1583-9, (1987) (PubMed).

  • Target
    CD14
    Andere Bezeichnung
    CD14 (CD14 Produkte)
    Synonyme
    CD14 molecule antikoerper, CD14 antigen antikoerper, CD14 antikoerper, Cd14 antikoerper
    Hintergrund
    CD14 Molecule,CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where it serves as a multifunctional lipopolysaccharide receptor. It is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.,LPS-receptor, LPSR, MCLR
    Gen-ID
    929
    UniProt
    P08571
    Pathways
    TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
Sie sind hier:
Kundenservice