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CD14 Antikörper (AA 20-214)

Name: CD14 Antikörper (AA 20-214)
Brand: antibodies-online
SKU: ABIN1724926
Price: 614.71 EUR
Availability: InStock

CD14 Reaktivität: Human, Maus FACS, WB, IHC, ELISA Wirt: Maus Monoclonal 4B4F12 unconjugated

Flow cytometric analysis of Jurkat cells using CD14 mouse mAb (green) and negative control (red).

FACS

Black line: Control Antigen (100 ng), Purple line: Antigen(10 ng), Blue line: Antigen (50 ng), Red line: Antigen (100 ng),

ELISA

Western blot analysis using CD14 mouse mAb against HepG2 (1), A549 (2), HL60 (3), RAW264.

Western blot analysis using CD14 mAb against human CD14 (AA: 20-214) recombinant protein.

Western blot analysis using CD14 mAb against HEK293 (1) and CD14 (AA: 20-214)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using CD14 mouse mAb with DAB staining.

IHC

6 Abbildungen

(2 Referenzen)

Produktnummer ABIN1724926

Datenblatt (PDF)

Target Alle CD14 Antikörper anzeigen

CD14
Bindungsspezifität
16

9

7

6

5

4

4

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 20-214
Reaktivität
267

54

32

30

29

28

25

24

24

18

17

14

10

9

8

8

8

5

2

2

2

1

1

1
Human, Maus
Wirt
224

63

25

3

1
Maus
Passende Sekundärantikörper
Klonalität
252

64
Monoklonal
Konjugat
127

30

24

18

15

8

6

6

5

5

5

4

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser CD14 Antikörper ist unkonjugiert
Applikation
223

102

63

59

52

49

22

22

21

14

14

13

7

6

4

4

3

3

3

3

3

3

2

2

2

2

1

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Aufreinigung

purified

Immunogen

Purified recombinant fragment of human CD14 (AA: 20-214) expressed in E. coli.

Klon

4B4F12

Isotyp

IgG1

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anti-CD14 (CD14) (AA 20-214) antibody
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Applikationshinweise

ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, FCM: 1:200 - 1:400

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Buffer

Purified antibody in PBS with 0.05 % sodium azide

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C/-20 °C

Informationen zur Lagerung

4°C, -20°C for long term storage
Matthews, Chung, Klasse, Moore, Sanders: "Potent induction of antibody-secreting B cells by human dermal-derived CD14+ dendritic cells triggered by dual TLR ligation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 12, pp. 5729-44, (2012) (PubMed).

Haghparast, Heidari Kharaji, Malvandi: "Down-regulation of CD14 transcripts in human glioblastoma cell line U87 MG." in: Iranian journal of immunology : IJI, Vol. 8, Issue 2, pp. 111-9, (2011) (PubMed).
Target

CD14

Andere Bezeichnung

CD14 (CD14 Produkte)

Synonyme

CD14 molecule antikoerper, CD14 antigen antikoerper, CD14 antikoerper, Cd14 antikoerper

Hintergrund

The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. , ,

Molekulargewicht

40 kDa

Gen-ID

929

HGNC

929

Pathways

TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades