Aktivieren Sie Javascript in Ihrem Browser, um unsere Webseite optimal zu nutzen.

CD14 Antikörper (Internal Region)

Name: CD14 Antikörper (Internal Region)
Brand: antibodies-online
SKU: ABIN185563
Price: 779.48 EUR
Availability: InStock

Verified CD14 Reaktivität: Human FACS, WB, ELISA Wirt: Ziege Polyclonal unconjugated

(ABIN185563) Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.

FACS

(ABIN185563) (2 μg/mL) staining of Human Lymph node (A) + peptide (B) and Tonsil (C) + peptide (D) lysate (35 μg protein in RIPA buffer) Detected by chemiluminescence.

ABIN185563 (1µg/ml) staining of Human Lymph Node lysate (35µg protein in RIPA buffer).

ABIN185563 (1µg/ml) staining of PBM lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide.

ABIN185563 (4µg/ml) staining of paraffin embedded Human Spleen.

IHC

5 Abbildungen

Produktnummer ABIN185563

Datenblatt (PDF)

Target Alle CD14 Antikörper anzeigen

CD14
Bindungsspezifität
16

9

7

6

5

4

4

3

3

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Internal Region
Reaktivität
268

55

32

30

29

28

25

24

24

18

17

14

10

9

8

8

8

5

2

2

2

1

1

1
Human
Wirt
225

64

25

2

1
Ziege
Passende Sekundärantikörper
Klonalität
254

63
Polyklonal
Konjugat
128

30

24

18

15

8

6

6

5

5

5

4

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser CD14 Antikörper ist unkonjugiert
Applikation
223

103

63

59

54

50

22

22

21

14

14

13

7

6

4

4

3

3

3

3

3

3

2

2

2

2

1

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), ELISA

Verwendungszweck

CD14

Sequenz

KRVDADADPR QYAD

Kreuzreaktivität

Human

Aufreinigung

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse

Verified

Immunogen

Peptide with sequence C-KRVDADADPRQYAD, from the internal region of the protein sequence according to NP_000582.1.

Isotyp

IgG

Top Product

Discover our top product CD14 Primärantikörper
anti-CD14 (CD14) (AA 54-83), (N-Term) antibody
CD14 Reaktivität: Human FACS, WB, IF, IHC (p) Wirt: Kaninchen Polyclonal RB14105 unconjugated

anti-CD14 (CD14) (AA 20-214) antibody
CD14 Reaktivität: Human WB, IHC, ELISA, ICC Wirt: Maus Monoclonal 1H5D8 unconjugated

anti-CD14 (CD14) (AA 20-214) antibody
CD14 Reaktivität: Human, Maus FACS, WB, IHC, ELISA Wirt: Maus Monoclonal 4B4F12 unconjugated

anti-CD14 (CD14) antibody
CD14 Reaktivität: Human IHC, ELISA Wirt: Maus Monoclonal 1A3B6 unconjugated

anti-CD14 (CD14) (AA 20-345) antibody
CD14 Reaktivität: Human FACS, WB, IHC, ELISA, IF Wirt: Maus Monoclonal 14G1F3 unconjugated

anti-CD14 (CD14) (AA 230-345) antibody
CD14 Reaktivität: Human FACS, WB, IHC, ELISA Wirt: Maus Monoclonal 1D1F8 unconjugated

anti-CD14 (CD14) (AA 25-148) antibody
CD14 Reaktivität: Human FACS, WB, IHC, ISt, StM Wirt: Maus Monoclonal LPSR-2385 unconjugated

anti-CD14 (CD14) antibody
CD14 Reaktivität: Human, Non-Human Primate FACS, WB, ELISA, IP Wirt: Maus Monoclonal MEM-18 unconjugated

anti-CD14 (CD14) antibody
CD14 Reaktivität: Human, Non-Human Primate FACS, WB, ELISA, IP, Func Wirt: Maus Monoclonal MEM-18 unconjugated

anti-CD14 (CD14) antibody (Biotin)
CD14 Reaktivität: Human, Non-Human Primate FACS, WB, ELISA, IP Wirt: Maus Monoclonal MEM-18 Biotin

Applikationshinweise

Western Blot: Approx 55 kDa band observed in Human Lymph node and Tonsil lysates (calculated MW of 40.1 kDa according to NP_000582.1).The observed molecular weight corresponds to the glycosylated form, and was successfully blocked by incubation with the im
Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

0.5 mg/mL

Buffer

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung

Minimize freezing and thawing.

Lagerung

-20 °C

Informationen zur Lagerung

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Target

CD14

Andere Bezeichnung

CD14 (CD14 Produkte)

Synonyme

CD14 molecule antikoerper, CD14 antigen antikoerper, CD14 antikoerper, Cd14 antikoerper

Hintergrund

CD14, CD14 antigen , HGNC:1628

Gen-ID

929

NCBI Accession

NP_000582

Pathways

TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades