Insulin Antikörper (HRP)

Produktnummer ABIN337134

Dieser Anti-Insulin Antikörper ist ein Maus Monoklonal Antikörper zur Detektion von Insulin in IHC, ELISA und IHC (p). Geeignet für Human.

Kurzübersicht für Insulin Antikörper (HRP) (ABIN337134)

Target: Insulin (INS)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Insulin Antikörper ist konjugiert mit HRP

Applikation: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: 8E2

Produktdetails anti-Insulin Antikörper (HRP)

Spezifität: Recognizes human Insulin. Species cross-reactivity: human proinsulin, bovine insulin (30 % ) and porcine insulin. Does not cross-react with free C-peptide.

Aufreinigung: Protein A purified

Immunogen: Purified human insulin.

Type of Immunogen: Purified protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, IHC, IHC-P (10 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Recommended as a detection antibody for ELISA and in sandwich assay with clone 3A6 as the capture antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.05 % Proclin 300

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid freeze-thaw cycles and prolonged exposure to light.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: store at 4°C. Long term: aliquot and store -20°C. Avoid freeze-thaw cycles. Protect from light.

Details zu Insulin

Target: Insulin (INS)

Andere Bezeichnung: Insulin

Hintergrund: Name/Gene ID: INS
Family: Hormone

Synonyms: INS, IRDN, IDDM2, Preproinsulin, ILPR, Insulin, MODY10, Proinsulin

Gen-ID: 3630

UniProt: P01308

Pathways: NF-kappaB Signalweg, RTK Signalweg, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagie, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation