p53 Antikörper

Produktnummer ABIN336891

Dieses Anti-p53-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von p53 in IHC und IHC (p). Geeignet für Human.

Kurzübersicht für p53 Antikörper (ABIN336891)

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser p53 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: P53-221

Produktdetails anti-p53 Antikörper

Spezifität: Molecular Weight: 53 kDa.

Immunogen: Recombinant human wild type p53 protein

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: IHC, ICC (frozen or formalin–fixed paraffin-embedded (FFPE) tissue sections, cell smears. For IHC dilute conc. antibodies 1:50-1:100, use streptavidin-biotin system or polymer system, incubate 30 minutes at room temperature. Prediluted antibody is ready to be used for IHC. FFPE tissue section requires antigen retriever (boiling tissue in 10 mM citrate, pH 6.0 for 20 mins, followed by cooling at RT for 20 mins). IF, FITC-conjugated antibodies 10-20 ug/ml (1:10-1:20), incubate for 2 hours in the dark at RT or it can also be incubated overnight at 4ºC. Flow: FITC-conjugated antibodies, 0.2-1.0 ug/0.1 ml (1:200-1:1,000) (Not tested in our lab). The optimum dilution should be determined by the individual lab. Positive Control: Human colon Cancer. Cellular Localization: Nuclear.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The antibodies are supplied with 1% BSA in PBS with 0.05% azide, pH 7.4. The Prediluted antibody is supplied in our Universal antibody dilution buffer (AR-6526) green in color. Description p53, also known as protein 53 (TP53), is a transcription factor th

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Details zu p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Andere Bezeichnung: p53

Pathways: p53 Signalweg, MAPK Signalweg, PI3K-Akt Signalweg, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagie, Warburg Effekt