p53 Antikörper (acLys315)

Produktnummer ABIN3201007

Produktdetails anti-p53 Antikörper

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Bindungsspezifität: acLys315

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser p53 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP)

Produktmerkmale: This product is the purified IgG fraction obtained from serum-free culture medium of mouse hybridoma (clone 10E5), which produces monoclonal antibody that specifically recognizes human p53 protein with acetylated Lys120.

Aufreinigung: Purified

Immunogen: synthetic peptide containing acLys315

Klon: 10E5

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: 1. Western blotting: ~1 g/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS ( pH 7.4), 50 % glycerol

Konservierungsmittel: Azide free

Lagerung: -20 °C

Informationen zur Lagerung: Upon arrival centrifuge briefly and store at -20 C.

Referenzen für anti-p53 Antikörper (ABIN3201007)

Tyteca, Legube, Trouche: "To die or not to die: a HAT trick." in: Molecular cell, Vol. 24, Issue 6, pp. 807-8, (2006) (PubMed).

Tang, Luo, Zhang, Gu: "Tip60-dependent acetylation of p53 modulates the decision between cell-cycle arrest and apoptosis." in: Molecular cell, Vol. 24, Issue 6, pp. 827-39, (2006) (PubMed).

Sykes, Mellert, Holbert, Li, Marmorstein, Lane, McMahon: "Acetylation of the p53 DNA-binding domain regulates apoptosis induction." in: Molecular cell, Vol. 24, Issue 6, pp. 841-51, (2006) (PubMed).

Details zu p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Andere Bezeichnung: p53 (TP53 Produkte)

Synonyme: BCC7 antikoerper, LFS1 antikoerper, P53 antikoerper, TRP53 antikoerper, p53 antikoerper, Trp53 antikoerper, brp53 antikoerper, drp53 antikoerper, etID22686.5 antikoerper, fb40d06 antikoerper, wu:fb40d06 antikoerper, zgc:111919 antikoerper, Tp53 antikoerper, bbl antikoerper, bfy antikoerper, bhy antikoerper, p44 antikoerper, tp53 antikoerper, Xp53 antikoerper, TP53 antikoerper, tumor protein p53 antikoerper, cellular tumor antigen p53 antikoerper, transformation related protein 53 antikoerper, tumor protein p53 L homeolog antikoerper, TP53 antikoerper, CpipJ_CPIJ002758 antikoerper, Tp53 antikoerper, tp53 antikoerper, Trp53 antikoerper, tp53.L antikoerper

Hintergrund: P53 mutants are found in more than half of human cancers and are considered as the most important human cancer related gene. p53 is detected at 53kD position by electrophoresis and is composed of 393 amino acids. In the unstressed normal cells, the p53 level is low and it is inactive. However, with stress, especially with DNA damage, it is activated to promote arrest of cell cycle and repair of DNA damage, or induction of apoptosis. The functions and stability of p53 are regulated by phosphorylation of serine and threonine, and acetylation of lysine at various sites in the molecule. Acetylation of lysine 120 (acetyl-K120) of p53 occurs rapidly after DNA damage and is catalyzed by the MYST family acetyltransferases hMOF and TIP60, and activates transcription of proapoptotic genes, BAX and PUMA.

UniProt: P04637

Pathways: p53 Signalweg, MAPK Signalweg, PI3K-Akt Signalweg, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagie, Warburg Effekt