p53 Antikörper (pSer315)

Produktnummer ABIN3201006

Der Maus Monoklonal Anti-p53-Antikörper wurde für WB, ELISA und IHC (p) validiert. Er ist geeignet, p53 in Proben von Human zu detektieren. Es sind 2+ Publikationen verfügbar.

Kurzübersicht für p53 Antikörper (pSer315) (ABIN3201006)

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser p53 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: 18

Produktdetails anti-p53 Antikörper

Bindungsspezifität: pSer315

Produktmerkmale: This product is the purified IgG fraction obtained from serum-free culture medium of mouse hybridoma (clone #18) which produces monoclonal antibody that specifically recognizes human p53 protein with phospholyrated Ser315.

Aufreinigung: Purified

Immunogen: Synthetic peptide containing pSer315 of p53

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: 1. Western blotting: 1,000~2000 dilution

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS ( pH 7.4), 50 % glycerol

Konservierungsmittel: Azide free

Lagerung: -20 °C

Informationen zur Lagerung: Upon arrival centrifuge briefly and store at -20 C.

Referenzen für anti-p53 Antikörper (ABIN3201006)

Bode, Dong: "Post-translational modification of p53 in tumorigenesis." in: Nature reviews. Cancer, Vol. 4, Issue 10, pp. 793-805, (2004) (PubMed).

Katayama, Sasai, Kawai, Yuan, Bondaruk, Suzuki, Fujii, Arlinghaus, Czerniak, Sen: "Phosphorylation by aurora kinase A induces Mdm2-mediated destabilization and inhibition of p53." in: Nature genetics, Vol. 36, Issue 1, pp. 55-62, (2003) (PubMed).

Details zu p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Andere Bezeichnung: p53

Hintergrund: P53 mutants are found in more than half of human cancers and are considered as the most important human cancer related gene. p53 is detected at 53kD position by electrophoresis and is composed of 393 amino acids. In the unstressed normal cells, the p53 level is low and it is inactive. However, with stress, especially with DNA damage, it is activated to promote arrest of cell cycle and repair of DNA damage, or induction of apoptosis. The functions and stability of p53 are regulated by phosphorylation of serine and threonine, and acetylation of lysine at various sites in the molecule. Ser315 is phosphorylated by aurora kinase and cycline-dependent kinases when cells are subjected to stress such as DNA damage and microtubule disruption by nocodazole. However the effect of the phosphorylation on the function of p53 is mostly unknown.

UniProt: P04637

Pathways: p53 Signalweg, MAPK Signalweg, PI3K-Akt Signalweg, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagie, Warburg Effekt