p53 Antikörper (full length)

Produktnummer ABIN306868

Dieser Maus Monoklonal Antikörper detektiert spezifisch p53 in WB, IP und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für p53 Antikörper (full length) (ABIN306868)

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser p53 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: BP53-12

Produktdetails anti-p53 Antikörper

Bindungsspezifität: AA 20-25, full length

Verwendungszweck: Mouse anti Human only p53 (a.a. 20-25)

Kreuzreaktivität: Human

Produktmerkmale: p53 is a 53 kDa transcription factor that can inhibit cell cycle progression or induce apoptosis in response to stress or DNA damage. Disruption of the p53 signalling pathway through various mechanisms is the most common alteration in human cancer occuring in over half of all tumors. The p53 protein is short lived and expressed at low levels in normal cells but accumulates and/or is activated in cells that have undergone genotoxic damage or oncogene activation. Many tumor derived and transformed cell lines express elevated levels of mutant p53 protein. Other genes also implicated in the downstream effects as a result of p53 activation are: p21WAF1, GADD45, 14-3-3, bax, Fas/APO1, KILLER/ DR5, Tsp1, IGF-BP3 and others.

Aufreinigung: Protein A/G Chromatography

Immunogen: Hybridoma produced by the fusion of splenocytes from immunized with full length wild-type human p53 protein and mouse myeloma cells.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: This antibody can be used for Western blotting (0.5-1 μg/mL), immunohistochemistry on formalin/paraffain embedded tissues (1-2 μg/mL) and immunoprecipitation (2 μg/mg of protein lysate). Antibody reacts with mutant as well as wild-type p53 protein. Optimal concentration should be evaluated by serial dilutions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Details zu p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Andere Bezeichnung: p53

UniProt: P04637

Pathways: p53 Signalweg, MAPK Signalweg, PI3K-Akt Signalweg, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagie, Warburg Effekt