p53 Antikörper (full length)

Produktnummer ABIN306867

Der Maus Monoklonal anti-p53 Antikörper (Klon Pab122) (ABIN306867) detektiert spezifisch p53 in IF, FACS und IP. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte, Affe und Hamster.

Kurzübersicht für p53 Antikörper (full length) (ABIN306867)

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Reaktivität: Human, Maus, Ratte, Affe, Hamster

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser p53 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP)

Klon: Pab122

Produktdetails anti-p53 Antikörper

Bindungsspezifität: AA 371-380, full length

Verwendungszweck: Mouse anti Human p53 (a.a. 371-380)

Kreuzreaktivität: Hamster, Human, Affe, Maus, Ratte

Produktmerkmale: p53 is a 53 kDa transcription factor that can inhibit cell cycle progression or induce apoptosis in response to stress or DNA damage. Disruption of the p53 signalling pathway through various mechanisms is the most common alteration in human cancer occuring in over half of all tumors. The p53 protein is short lived and expressed at low levels in normal cells but accumulates and/or is activated in cells that have undergone genotoxic damage or oncogene activation. Many tumor derived and transformed cell lines express elevated levels of mutant p53 protein. Other genes also implicated in the downstream effects as a result of p53 activation are: p21WAF1, GADD45, 14-3-3, bax, Fas/APO1, KILLER/ DR5, Tsp1, IGF-BP3 and others.

Aufreinigung: Protein A/G Chromatography

Immunogen: Hybridoma produced by the fusion of splenocytes from immunized with full length mouse p53 protein and mouse myeloma cells.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: This antibody can be used for Western blotting (5-10 μg/mL), immunohistochemistry on frozen and formalin/paraffain embedded tissues (1-2 μg/mL) and immunoprecipitation (2 μg/mg of protein lysate). Optimal concentration should be evaluated by serial dilutions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Details zu p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Andere Bezeichnung: p53

UniProt: Q9BRQ8

Pathways: p53 Signalweg, MAPK Signalweg, PI3K-Akt Signalweg, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagie, Warburg Effekt