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MAP2K7 Antikörper (N-Term)

MAP2K7 Reaktivität: Human, Maus, Ratte, Affe WB, IHC, IF, ICC, FACS Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN3043875
  • Target Alle MAP2K7 Antikörper anzeigen
    MAP2K7 (Mitogen-Activated Protein Kinase Kinase 7 (MAP2K7))
    Bindungsspezifität
    • 35
    • 22
    • 8
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 2-40, N-Term
    Reaktivität
    • 79
    • 48
    • 47
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Maus, Ratte, Affe
    Wirt
    • 89
    • 5
    Kaninchen
    Klonalität
    • 81
    • 13
    Polyklonal
    Konjugat
    • 32
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Dieser MAP2K7 Antikörper ist unkonjugiert
    Applikation
    • 64
    • 27
    • 25
    • 25
    • 15
    • 13
    • 8
    • 6
    • 6
    • 5
    • 4
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
    Verwendungszweck
    Anti-MEK7/MAP2K7 Antibody Picoband®
    Sequenz
    AASSLEQKLS RLEAKLKQEN REARRRIDLN LDISPQRPR
    Kreuzreaktivität (Details)
    No cross-reactivity with other proteins.
    Produktmerkmale
    Anti-MEK7/MAP2K7 Antibody Picoband® (ABIN3043875). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
    Aufreinigung
    Immunogen affinity purified.
    Immunogen
    A synthetic peptide corresponding to a sequence at the N-terminus of human MEK7, identical to the related mouse and rat sequences.
    Isotyp
    IgG
    Top Product
    Discover our top product MAP2K7 Primärantikörper
  • Applikationshinweise
    Western blot, 0.1-0.25 μg/mL, Human, Monkey, Mouse, Rat
    Immunohistochemistry (Paraffin-embedded Section), 1-2 μg/mL, Human
    Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
    1. Lu, X., Nemoto, S., Lin, A. Identification of c-jun NH(2)-terminal protein kinase (JNK)-activating kinase 2 as an activator of JNK but not p38. J. Biol. Chem. 272: 24751-24754, 1997. 2. Schramek, D., Kotsinas, A., Meixner, A., Wada, T., Elling, U., Pospisilik, J. A., Neely, G. G., Zwick, R.-H., Sigl, V., Forni, G., Serrano, M., Gorgoulis, V. G., Penninger, J. M. The stress kinase MKK7 couples oncogenic stress to p53 stability and tumor suppression. Nature Genet. 43: 212-219, 2011. 3. Wu, Z., Wu, J., Jacinto, E., Karin, M. Molecular cloning and characterization of human JNKK2, a novel jun NH(2)-terminal kinase-specific kinase. Molec. Cell. Biol. 17: 7407-7416, 1997.
    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Konzentration
    500 μg/mL
    Buffer
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Target
    MAP2K7 (Mitogen-Activated Protein Kinase Kinase 7 (MAP2K7))
    Andere Bezeichnung
    MAP2K7 (MAP2K7 Produkte)
    Hintergrund

    Synonyms: Dual specificity mitogen-activated protein kinase kinase 7;MAP kinase kinase 7;MAPKK 7;2.7.12.2;JNK-activating kinase 2;MAPK/ERK kinase 7;MEK 7;Stress-activated protein kinase kinase 4;SAPK kinase 4;SAPKK-4;SAPKK4;c-Jun N-terminal kinase kinase 2;JNK kinase 2;JNKK 2;MAP2K7;JNKK2, MEK7, MKK7, PRKMK7, SKK4;

    Tissue Specificity: Ubiquitous; with highest level of expression in skeletal muscle. Isoform 3 is found at low levels in placenta, fetal liver, and skeletal muscle. .

    Background: MAP2K7 (Mitogen-activated protein kinase kinase 7), also known as MAP kinase kinase 7, MAPKK7, JNKK2, PRKMK7 or MKK7, is an enzyme that in humans is encoded by the MAP2K7 gene. This protein is a member of the mitogen-activated protein kinase kinase family. The MKK7 protein exists as six different isoforms with three possible N-termini (α, β, and γ isoforms) and two possible C-termini (1 and 2 isoforms). Schramek et al. (2011) showed that the doxorubicin-mediated DNA damage response in human A549 lung carcinoma cells caused rapid phosphorylation and upregulation of p53 (TP53). MKK7 knockdown reduced p53 phosphorylation, delayed p53 upregulation, and interfered with cell cycle arrest at G2/M. MKK7 was activated in primary lung tumors, and tumors with a p53 mutation showed even higher MKK7 phosphorylation. Schramek et al. (2011) concluded that MKK7 exerts its tumor suppressive function through p53.

    Molekulargewicht
    47 kDa
    Gen-ID
    5609
    UniProt
    O14733
    Pathways
    MAPK Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Activation of Innate immune Response, Toll-Like Receptors Cascades, BCR Signaling
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