PKC alpha Antikörper (N-Term)

Produktnummer ABIN3043025

Der Kaninchen Polyklonal Anti-PKC alpha-Antikörper wurde für WB und IHC validiert. Er ist geeignet, PKC alpha in Proben von Human, Maus und Ratte zu detektieren. Es sind 5+ Publikationen verfügbar.

Kurzübersicht für PKC alpha Antikörper (N-Term) (ABIN3043025)

Target: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PKC alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-PKC alpha Antikörper

Bindungsspezifität: AA 153-182, N-Term

Verwendungszweck: Anti-PKC alpha/PRKCA Antibody Picoband®

Sequenz: MDHTEKRGRI YLKAEVADEK LHVTVRDAKN

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-PKC alpha/PRKCA Antibody (ABIN3043025). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human PRKCA, different from the related rat and mouse sequences by one amino acid.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Braz, J. C., Gregory, K., Pathak, A., Zhao, W., Sahin, B., Klevitsky, R., Kimball, T. F., Lorenz, J. N., Nairn, A. C., Liggett, S. B., Bodi, I., Wang, S., and 9 others : PKC-alpha regulates cardiac contractility and propensity toward heart failure. Nature Med. 10: 248-254, 2004. 2. Latos-Bielenska, A., Klett, C., Just, W., Hameister, H. : Refinement of localization of the human genes for myeloperoxidase (MPO), protein kinase C, alpha polypeptide, PRKCA, and the DNA fragment D17S21 on chromosome 17q. Hereditas 115: 69-72, 1991.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-PKC alpha Antikörper (ABIN3043025)

Yan-Fang, Zhi-Heng, Li-Xiao, Fang, Jun, Gang, Lan, Na-Na, Xiao-Juan, Li-Chao, Wen-Li, Pei-Fang, He, Guang-Hao, Yan-Hong, Yi-Ping, Yun-Yun, Hui-Ting, Yi, Mei-Fang, Lin, Jian, Shao-Yan, Xue-Ming, Xing et al.: "Molecular Mechanism of the Cell Death Induced by the Histone Deacetylase Pan Inhibitor LBH589 (Panobinostat) in Wilms Tumor Cells. ..." in: PLoS ONE, Vol. 10, Issue 7, pp. e0126566, (2016) (PubMed).

Gan, Wang, Zhao, Wu, Yang, Peng: "5-(Bis(3-(2-hydroxyethyl)-1H-indol-2-yl)methyl)-2-hydroxybenzoic acid (BHIMHA): showing a strategy of designing drug to block lung metastasis of tumors." in: Drug design, development and therapy, Vol. 10, pp. 711-21, (2016) (PubMed).

Cheng, Gu, Yuan, Feng, Jia: "Suppression of A549 cell proliferation and metastasis by calycosin via inhibition of the PKC-α/ERK1/2 pathway: An in vitro investigation." in: Molecular medicine reports, Vol. 13, Issue 4, pp. 3709-10, (2016) (PubMed).

Sun, Li, Ma, Liu, Xu, Ye, Hou, Wang, Li, Jiang: "The predictive value and potential mechanisms of miRNA-328 and miRNA-378 for brain metastases in operable and advanced non-small-cell lung cancer." in: Japanese journal of clinical oncology, Vol. 45, Issue 5, pp. 464-73, (2015) (PubMed).

Cheng, Gu, Zhang, Yuan, Zhao, Jiang, Jia: "Astragaloside IV inhibits migration and invasion in human lung cancer A549 cells via regulating PKC-?-ERK1/2-NF-?B pathway." in: International immunopharmacology, Vol. 23, Issue 1, pp. 304-13, (2015) (PubMed).

Details zu PKC alpha

Target: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Andere Bezeichnung: PRKCA

Hintergrund:

Synonyms: Protein kinase C alpha type,PKC-A,PKC-alpha,2.7.11.13,PRKCA,PKCA, PRKACA,

Tissue Specificity: Brain and peripheral tissues.

Background: Protein kinase C (PKC) is the major phorbol ester receptor. Activation of PKC by calcium ions and the second messenger diacylglycerol is though to play a central role in the induction of cellular responses to a variety of ligand-receptor systems and in the regulation of cellular responsiveness to external stimuli. Three of these, termed alpha, beta and gamma, are highly homologous. PRKCA1 is mapped to 17q22-q23.2. PKC-alpha regulates cardiac contractility and propensity toward heart failure.

Sequence Similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

Molekulargewicht: 77 kDa

UniProt: P17252

Pathways: WNT Signalweg, T-Zell Rezeptor Signalweg, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling