p53 Antikörper (AA 37-45)

Produktnummer ABIN3025573

Der Maus Monoklonal anti-p53 Antikörper (Klon SPM590) (ABIN3025573) detektiert spezifisch p53 in WB, IF, FACS und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Affe und Rind (Kuh).

Kurzübersicht für p53 Antikörper (AA 37-45) (ABIN3025573)

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Reaktivität: Human, Affe, Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser p53 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: SPM590

Produktdetails anti-p53 Antikörper

Bindungsspezifität: AA 37-45

Aufreinigung: Protein G affinity chromatography

Immunogen: Recombinant human wild type p53 protein was used as the immunogen for this anti-p53 antibody. Its epitope maps within the N-terminus (approx. amino acids 37-45).

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: The optimal dilution of the anti-p53 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 minutes at RT (1),Prediluted format: incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the anti-p53 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Andere Bezeichnung: p53

Hintergrund: Recognizes a 53  kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.

Pathways: p53 Signalweg, MAPK Signalweg, PI3K-Akt Signalweg, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagie, Warburg Effekt