IL-1 beta Antikörper (Biotin)

Produktnummer ABIN288401

Der Ziege Polyklonal Anti-IL-1 beta-Antikörper wurde für WB, IHC und ELISA validiert. Er ist geeignet, IL-1 beta in Proben von Human zu detektieren.

Kurzübersicht für IL-1 beta Antikörper (Biotin) (ABIN288401)

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser IL-1 beta Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Produktdetails anti-IL-1 beta Antikörper (Biotin)

Sterilität: Filtered

Immunogen: IL1 b antibody (biotin) was raised in goat using E. Coli derived recombinant human IL1?/IL1 2 as the immunogen.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 2-8 µg/mL, IHC: 515 µg/mL, WB: 0.1 µg/mL
Optimal conditions should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute to a concentration of 50 µg/mL with 1 mL of sterile 20 mM Trizma Base, 150 mM NaCl, pH 7.3 containing 0.1 % BSA.

Konzentration: Lot specific

Buffer: Lyophilized from sterile filtered PBS solution with BSA.

Handhabung: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at -20 °C until reconstitution. Following reconstitution product may be stored at 4 °C in the short term. For long term storage aliquot and freeze at -20 °C.

Details zu IL-1 beta

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Andere Bezeichnung: IL1b

Hintergrund: IL1 is a name that designates two pleiotropic cytokines, IL1 alpha (IL1F1) and IL1Beta (IL1F2), which are the products of distinct genes. IL1 alpha and IL1Beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL1 alpha and IL1Beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL1 RI binds directly to IL1 alpha or IL1Beta and then associates with IL1 R accessory protein (IL1 R3/IL1 R AcP) to form a highaffinity receptor complex that is competent for signal transduction. IL1 RII has high affinity for IL1Beta but functions as a decoy receptor and negative regulator of IL1Beta activity.

Pathways: NF-kappaB Signalweg, Interferon-gamma Pathway, TLR Signalweg, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagie, Cancer Immune Checkpoints, Inflammasome