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p53 Antikörper

Der Kaninchen Polyklonal Anti-p53-Antikörper wurde für WB, IF, IHC (p), IP, ICC und ChIP validiert. Er ist geeignet, p53 in Proben von Human zu detektieren. Es ist 1 Publikation verfügbar.
Produktnummer ABIN2855525

Kurzübersicht für p53 Antikörper (ABIN2855525)

Target

Alle p53 (TP53) Antikörper anzeigen
p53 (TP53) (Tumor Protein P53 (TP53))

Reaktivität

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Human

Wirt

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Kaninchen

Klonalität

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Polyklonal

Konjugat

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Dieser p53 Antikörper ist unkonjugiert

Applikation

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Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP)

Güteklasse

KO Validated
  • Kreuzreaktivität

    Human, Maus, Zebrafisch (Danio rerio)

    Produktmerkmale

    Rabbit Polyclonal antibody to p53 (tumor protein p53)
    p53 antibody

    Aufreinigung

    Purified by antigen-affinity chromatography.

    Immunogen

    Recombinant protein encompassing a sequence within the center region of human p53. The exact sequence is proprietary.

    Isotyp

    IgG
  • Applikationshinweise

    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

    Kommentare

    Positive Control: HCT116 cells with 30uM cisplatin treatment for 24hr

    Validation: KO/KD, Orthogonal

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    1.28 mg/mL

    Buffer

    1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

    Konservierungsmittel

    ProClin

    Vorsichtsmaßnahmen

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Chen, Lin, Liang, Villalobos, Villaflores, Lou, Lai, Hsiao: "UVB Irradiation Induced Cell Damage and Early Onset of Junbb Expression in Zebrafish." in: Animals : an open access journal from MDPI, Vol. 10, Issue 6, (2020) (PubMed).

  • Target

    p53 (TP53) (Tumor Protein P53 (TP53))

    Andere Bezeichnung

    tumor protein p53

    Hintergrund

    This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity.

    Cellular Localization: Cytoplasm , Nucleus , Endoplasmic reticulum

    Molekulargewicht

    44 kDa

    Gen-ID

    7157

    UniProt

    P04637

    Pathways

    p53 Signalweg, MAPK Signalweg, PI3K-Akt Signalweg, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagie, Warburg Effekt
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