GBL Antikörper (Middle Region)

Produktnummer ABIN2787309

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch GBL in WB. Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte, Rind (Kuh), Hund, Ziege, Meerschweinchen, Kaninchen, Pferd und Zebrafisch (Danio rerio).

Kurzübersicht für GBL Antikörper (Middle Region) (ABIN2787309)

Target: GBL (G protein beta subunit-like (GBL))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Ziege, Meerschweinchen, Kaninchen, Pferd, Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GBL Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-GBL Antikörper

Bindungsspezifität: Middle Region

Verwendungszweck: GBL Antibody - middle region

Sequenz: CAFSGDSQYI VTASSDNLAR LWCVETGEIK REYGGHQKAV VCLAFNDSVL

Homologie: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Produktmerkmale: This is a rabbit polyclonal antibody against GBL. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human GBL

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP57630-100UG

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: prevent freeze-thaw cycles

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Details zu GBL

Target: GBL (G protein beta subunit-like (GBL))

Andere Bezeichnung: GBL

Hintergrund:

Targeted Subunit: LST8

Background Information: GBL is the subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.

Gene Name: MTOR associated protein, LST8 homolog (S. cerevisiae)

Alternative Symbols: GBL, LST8, POP3, WAT1, GbetaL

Protein Name: Target of rapamycin complex subunit LST8

Molekulargewicht: 36kDa

Gen-ID: 64223

NCBI Accession: NP_071767

UniProt: Q9BVC4

Pathways: PI3K-Akt Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Actin Filament Polymerization, Autophagie, CXCR4-mediated Signaling Events, BCR Signaling, Warburg Effekt