Bcl-2 Antikörper (AA 41-54)

Produktnummer ABIN2738523

Der Maus Monoklonal anti-Bcl-2 Antikörper wird verwendet zum Nachweis von Bcl-2 in Proben von Human, Schwein, Affe, Hund und Schimpanse. Er wurde validiert für WB, IHC, FACS, IF, ELISA, IP und IHC (p).

Kurzübersicht für Bcl-2 Antikörper (AA 41-54) (ABIN2738523)

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Reaktivität: Human, Schwein, Affe, Hund, Schimpanse

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Bcl-2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: 8C8

Produktdetails anti-Bcl-2 Antikörper

Bindungsspezifität: AA 41-54

Spezifität: Human BCL2 / Bcl-2

Keine Kreuzreaktivität: Maus, Ratte (Rattus)

Aufreinigung: Protein G purified

Immunogen: A synthetic peptide, aa41-54 (GaaPAPGIFSSQPG-Cys) of human Bcl-2 protein. Percent identity by BLAST analysis: Human, Chimpanzee, Cat, Pig, Dog (100%), Gorilla, Orangutan, Bovine, Goat (91%), Galago (82%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, Flo (0.5 - 1 μg/10 x10⁶), IF (1 - 2 μg/mL), IHC, IHC-P (0.5 - 1 μg/mL), IP, WB (0.5 - 1 μg/mL)

Usage: This antibody recognizes a protein of 25-26  kDa, identified as the Bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of Bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of Bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express Bcl-2 protein and the small number in which the neoplastic cells are Bcl-2 negative. ELISA: Order BSA-free format for coating.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 mg/mL BSA, 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2°C to 8°C.

Details zu Bcl-2

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Andere Bezeichnung: BCL2 / Bcl-2

Hintergrund: Name/Gene ID: BCL2
Family: Apoptosis

Synonyms: BCL2, Apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, B-cell lymphoma protein 2, Bcl-2, PPP1R50

Gen-ID: 596

Pathways: MAPK Signalweg, PI3K-Akt Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagie, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling