Phospholipase C gamma 2 Antikörper

Produktnummer ABIN272070

Der Kaninchen Polyklonal anti-Phospholipase C gamma 2 Antikörper (ABIN272070) detektiert spezifisch Phospholipase C gamma 2 in WB und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Ratte und Maus.

Kurzübersicht für Phospholipase C gamma 2 Antikörper (ABIN272070)

Target: Phospholipase C gamma 2 (PLCG2)

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Phospholipase C gamma 2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Phospholipase C gamma 2 Antikörper

Spezifität: This antibody detects endogenous levels of PLCG2 protein. (region surrounding asn1211)

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatography using epitope-specific immunogen.

Anwendungsinformationen

Applikationshinweise: ELISA: 1/5000-1/10000. Western blot 1/500-1/1000. Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 0.05 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Phospholipase C gamma 2

Target: Phospholipase C gamma 2 (PLCG2)

Andere Bezeichnung: PLCG2

Hintergrund: Phosphoinositide-specific phospholipase C (PLC) plays a crucial role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1,4,5-triphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. There are many mammalian PLC isozymes, including PLC β1, PLC β2, PLC β3, PLC β4, PLC γ1, PLC γ2, PLC δ1, PLC δ2 and PLC ε). PLC βs are the only PLC isforms that are regulated by G protein subunits and are activated by a heterotrimeric GTP-binding protein linked to various cell surface receptors. Two alternatively spliced forms (1181 and 1166 amino acids) of PLC β2 are generated in haematopoietic cells that differ in the carboxyl-terminal sequence implicated in interaction of PLC β enzymes with G αq. The Pleckstrin homology domain of PLC β2 is required for its targeting to the membrane and for substrate hydrolysis and its linker region exerts an inhibitory effect on basal PLC β2 activity. PLC β2 plays a major role in platelet activation and is mainly expressed in the periphery of the islet and acinar cells in rat pancreas.Synonyms: 1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase gamma-2, PLC-IV, PLC-gamma-2, Phosphoinositide phospholipase C-gamma-2, Phospholipase C-IV, Phospholipase C-gamma-2

Molekulargewicht: approx. 148 kDa

Gen-ID: 5336

NCBI Accession: NP_002652

UniProt: P16885

Pathways: RTK Signalweg, WNT Signalweg, Fc-epsilon Rezeptor Signalübertragung, Inositol Metabolic Process, Myometrial Relaxation and Contraction, Toll-Like Receptors Cascades, VEGF Signaling, BCR Signaling