p53 Antikörper (Alexa Fluor 647)

Produktnummer ABIN2689872

Produktdetails anti-p53 Antikörper (Alexa Fluor 647)

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser p53 Antikörper ist konjugiert mit Alexa Fluor 647

Applikation: Intracellular Staining (ICS)

Marke: BD Phosflow™

Produktmerkmale: The p53 protein is critical to regulation of normal cell growth and proliferation and is a suppressor of tumor cell proliferation. Inactivation of p53 by a number of mechanisms, such as missense mutations or interaction with oncogenic viral or cellular proteins, can result in tumor progression. Mutations and/or allelic loss of the p53 gene are associated with a wide variety of human tumors. Known to have a role in transcriptional regulation, p53 suppresses various promoters containing TATA elements in an apparently sequence-independent fashion. p53 also binds to DNA in a sequence-specific manner via recognition of a 20-bp consensus-binding site. This interaction stimulates the expression of genes downstream of the p53 binding site. A number of genes that contain p53-binding sites have been identified, including MDM2, GADD45, and muscle creatine kinase. Post-translational acetylation of p53 enhances its DNA-binding activity. There are multiple factors that affect p53 acetylation, thereby modulating cellular proliferation and apoptosis. The L82-51 monoclonal antibody recognizes acetylated lysine 382 (acK382) in the C-terminal region of p53. Analysis of p53 (acK382) in lymphocytes. Human peripheral blood mononuclear cells (PBMC) were either treated with 0.4 μM Trichostatin A (Sigma, Cat. No. T8552) plus 0.4 μM Adriamycin (Doxorubicin hydrochloride, Sigma, Cat. No. D1515) for 24 hours (shaded histogram) or untreated (open histogram). The cells were fixed (BD Cytofix™ buffer, Cat. No. 554655) for 10 minutes, then permeabilized (BD Phosflow™ Perm Buffer III, Cat. No. 558050) on ice for at least 30 minutes, and then stained with Alexa Fluor® 647 Mouse anti-p53 (acK382). Lymphocytes were selected by scatter profile. Flow cytometry was performed on a BD FACSArray™ bioanalyzer system. The specificity of mAb L82-51 was confirmed by western blot analysis using unconjugated antibody on lysates from control (left blot) and Trichostatin A-plus-Adriamycin-treated (right blot) PBMC. p53 (acK382) is identified as a band of 53 kDa in the treated cells.

BD Phosflow™ Alexa Fluor® 647 Mouse anti-p53 (acK382) - Alexa Fluor® 647 - Clone L82-51 - Isotype Mouse IgG1, κ - Reactivity Hu - 50 Tests

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human p53 acetylated Peptide

Klon: L82

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Probenmenge: 20 μL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Referenzen für anti-p53 Antikörper (Alexa Fluor 647) (ABIN2689872)

Nishino, Wang, Mochizuki-Kashio, Osawa, Nakauchi, Iwama: "Ex vivo expansion of human hematopoietic stem cells by garcinol, a potent inhibitor of histone acetyltransferase." in: PLoS ONE, Vol. 6, Issue 9, pp. e24298, (2011) (PubMed).

Soliman, Riabowol: "After a decade of study-ING, a PHD for a versatile family of proteins." in: Trends in biochemical sciences, Vol. 32, Issue 11, pp. 509-19, (2007) (PubMed).

Solomon, Pasupuleti, Xu, McDonagh, Curtis, DiStefano, Huber: "Inhibition of SIRT1 catalytic activity increases p53 acetylation but does not alter cell survival following DNA damage." in: Molecular and cellular biology, Vol. 26, Issue 1, pp. 28-38, (2005) (PubMed).

Pedeux, Sengupta, Shen, Demidov, Saito, Onogi, Kumamoto, Wincovitch, Garfield, McMenamin, Nagashima, Grossman, Appella, Harris: "ING2 regulates the onset of replicative senescence by induction of p300-dependent p53 acetylation." in: Molecular and cellular biology, Vol. 25, Issue 15, pp. 6639-48, (2005) (PubMed).

Hofmann, Möller, Sirma, Zentgraf, Taya, Dröge, Will, Schmitz: "Regulation of p53 activity by its interaction with homeodomain-interacting protein kinase-2." in: Nature cell biology, Vol. 4, Issue 1, pp. 1-10, (2002) (PubMed).

Saito, Goodarzi, Higashimoto, Noda, Lees-Miller, Appella, Anderson: "ATM mediates phosphorylation at multiple p53 sites, including Ser(46), in response to ionizing radiation." in: The Journal of biological chemistry, Vol. 277, Issue 15, pp. 12491-4, (2002) (PubMed).

Ito, Lai, Zhao, Saito, Hamilton, Appella, Yao: "p300/CBP-mediated p53 acetylation is commonly induced by p53-activating agents and inhibited by MDM2." in: The EMBO journal, Vol. 20, Issue 6, pp. 1331-40, (2001) (PubMed).

Vaziri, Dessain, Ng Eaton, Imai, Frye, Pandita, Guarente, Weinberg: "hSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase." in: Cell, Vol. 107, Issue 2, pp. 149-59, (2001) (PubMed).

Details zu p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Andere Bezeichnung: p53 (TP53 Produkte)

Synonyme: BCC7 antikoerper, LFS1 antikoerper, P53 antikoerper, TRP53 antikoerper, p53 antikoerper, Trp53 antikoerper, brp53 antikoerper, drp53 antikoerper, etID22686.5 antikoerper, fb40d06 antikoerper, wu:fb40d06 antikoerper, zgc:111919 antikoerper, Tp53 antikoerper, bbl antikoerper, bfy antikoerper, bhy antikoerper, p44 antikoerper, tp53 antikoerper, Xp53 antikoerper, TP53 antikoerper, tumor protein p53 antikoerper, cellular tumor antigen p53 antikoerper, transformation related protein 53 antikoerper, tumor protein p53 L homeolog antikoerper, TP53 antikoerper, CpipJ_CPIJ002758 antikoerper, Tp53 antikoerper, tp53 antikoerper, Trp53 antikoerper, tp53.L antikoerper

Hintergrund: Synonyms: TP53 (acK382)

Pathways: p53 Signalweg, MAPK Signalweg, PI3K-Akt Signalweg, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagie, Warburg Effekt