IL12 Antikörper (Biotin)

Produktnummer ABIN2689732

Produktdetails anti-IL12 Antikörper (Biotin)

Target: IL12 (Interleukin 12 (IL12))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser IL12 Antikörper ist konjugiert mit Biotin

Applikation: ELISA

Marke: BD Pharmingen™

Produktmerkmale: The C8.6 antibody reacts with human IL-12 p40 monomers, homodimers, and p70 heterodimers, but not p35 subunits. The immunogen used to generate the C8.6 hybridoma was the purified p40 subunit of human IL-12. The C8.6 antibody is a neutralizing antibody and has been reported to strongly inhibit three different biological activities of IL-12 (i.e., IFN-γ induction), mitogenic effects on PHA blasts, and enhancement of NK cell-mediated cytotoxicity. C8.6 has been shown to immunoprecipitate IL-23. This antibody is routinely tested by ELISA as a detection antibody. Other applications were tested during antibody development only or reported in the literature. Human IL-12 p70 Protein Quantitation by ELISA. Rat anti-human IL-12 p70 specific antibody 20C2 (Cat. No. 555065) was paired with a biotinylated anti-IL-12 p40/p70 detection (Cat. No. 554660) to measure specifically human IL-12 p70 protein, using recombinant human IL-12 p70 (Cat. No. 554613), as the ELISA standard.

BD Pharmingen™ Biotin Mouse Anti-Human IL-12 (p40/p70) - Biotin - Clone C8.6 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.5 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Purified p40 Subunit of Human IL-12

Klon: C8-6

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Referenzen für anti-IL12 Antikörper (Biotin) (ABIN2689732)

Oppmann, Lesley, Blom, Timans, Xu, Hunte, Vega, Yu, Wang, Singh, Zonin, Vaisberg, Churakova, Liu, Gorman, Wagner, Zurawski, Liu, Abrams, Moore, Rennick, de Waal-Malefyt, Hannum, Bazan, Kastelein: "Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12." in: Immunity, Vol. 13, Issue 5, pp. 715-25, (2001) (PubMed).

DAndrea, Rengaraju, Valiante, Chehimi, Kubin, Aste, Chan, Kobayashi, Young, Nickbarg: "Production of natural killer cell stimulatory factor (interleukin 12) by peripheral blood mononuclear cells." in: The Journal of experimental medicine, Vol. 176, Issue 5, pp. 1387-98, (1992) (PubMed).

Details zu IL12

Target: IL12 (Interleukin 12 (IL12))

Andere Bezeichnung: IL-12 p40,p70 (IL12 Produkte)

Synonyme: CLMF antikoerper, CLMF2 antikoerper, IL-12B antikoerper, NKSF antikoerper, NKSF2 antikoerper, p40 antikoerper, Il-12b antikoerper, Il12p40 antikoerper, Il-12p40 antikoerper, Il12 antikoerper, IL-12p40 antikoerper, IL-12 antikoerper, LOC100217488 antikoerper, IL-12p35 antikoerper, Il-12a antikoerper, Ll12a antikoerper, p35 antikoerper, IL12p40 antikoerper, IL12B antikoerper, IL-12 p35 antikoerper, interleukin 12B antikoerper, interleukin 12A antikoerper, interleukin 12b antikoerper, interleukin 12a antikoerper, Interleukin 12 level antikoerper, IL12B antikoerper, IL12A antikoerper, Il12b antikoerper, Il12a antikoerper, IL12 antikoerper, il12a antikoerper

Pathways: JAK-STAT Signalweg, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome