p53 Antikörper (AA 371-380)

Produktnummer ABIN264449

Der Maus Monoklonal Anti-p53-Antikörper wurde für WB, IP, IHC (fro) und EIA validiert. Er ist geeignet, p53 in Proben von Human, Maus, Ratte und Affe zu detektieren.

Kurzübersicht für p53 Antikörper (AA 371-380) (ABIN264449)

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Reaktivität: Human, Maus, Ratte, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser p53 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Klon: HR231

Produktdetails anti-p53 Antikörper

Bindungsspezifität: AA 371-380

Spezifität: This antibody reacts to p53.

Kreuzreaktivität (Details): Species reactivity (tested):Human, Monkey, Mouse and Rat.

Produktmerkmale: Synonyms: Cellular tumor antigen p53, Tumor suppressor p53, Phosphoprotein p53, NY-CO-13

Aufreinigung: Affinity chromatography on Protein A/G

Immunogen: Hybridoma produced by the fusion of splenocytes from immunized with full length humanp53 protein and mouse myeloma cells.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Western Blot (1-5 μg/mL). ELISA. Immunoprecipitation. Immunohistochemistry. Positive Control: Breast or colon carcinoma cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: PBS, 0.08 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Haltbarkeit: 12 months

Details zu p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Andere Bezeichnung: p53 (TP53)

Hintergrund: P53 is a 53 kDa transcription factor that can inhibit cell cycle progression or induce apoptosis in response to stress or DNA damage. Disruption of the p53 signalling pathway through various mechanisms is the most common alteration in human cancer occuring in over half of all tumors. The p53 protein is short lived and expressed at low levels in normal cells but accumulates and/or is activated in cells that have undergone genotoxic damage or oncogene activation. Many tumor derived and transformed cell lines express elevated levels of mutant p53 protein. Other genes also implicated in the downstream effects as a result of p53 activation are: p21WAF1, GADD45, 14-3-3, bax, Fas/APO1, KILLER/ DR5, Tsp1, IGF-BP3 and others.Synonyms: Cellular tumor antigen p53, NY-CO-13, Phosphoprotein p53, Tumor suppressor p53

Gen-ID: 7157

UniProt: P04637

Pathways: p53 Signalweg, MAPK Signalweg, PI3K-Akt Signalweg, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagie, Warburg Effekt