Insulin Antikörper

Produktnummer ABIN2624251

Dieses Anti-Insulin-Antikörper ist ein Meerschweinchen Polyklonal-Antikörper zur Detektion von Insulin in ELISA, IF und DB. Geeignet für Schwein.

Kurzübersicht für Insulin Antikörper (ABIN2624251)

Target: Insulin (INS)

Reaktivität: Schwein

Wirt: Meerschweinchen

Klonalität: Polyklonal

Konjugat: Dieser Insulin Antikörper ist unkonjugiert

Applikation: ELISA, Immunofluorescence (IF), Dot Blot (DB)

Produktdetails anti-Insulin Antikörper

Spezifität: Swine insulin

Aufreinigung: Purified IgG

Immunogen: Insulin is a hormone produced by the beta cells of the islets of Langerhans in the pancreas. It plays an important role in the metabolism of carbohydrate by its effects on the storage of glycogen in the liver, the conversion of carbohydrate to fat, and the oxidation of glucose by the peripheral tissues. Insulin has a molecular weight of 6,000 and contains two peptide chains linked by two disulphide bonds. The hormone is isolated from porcine pancreas. Freund's complete adjuvant is used in the fi

Type of Immunogen: Purified protein

Anwendungsinformationen

Applikationshinweise: Approved: DB, ELISA, IF

Usage: The reactivity of the antiserum is directed to insulin as tested by ELISA and immunoblotting. No reaction was obtained with other swine serum proteins. The cytochemical grade allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates. in radioimmunoassay. for the production of immunoconjugates with a selected marker. to prepare immunoaffinity adsorbents by coupling to an artificial carrier. in non-isotopic methodology based on solid phase immunochemistry (e. g. ELISA), both as catching antibody and detection reagent. in Western blotting. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:250. in ELISA and comparable non-precipitating antibody-binding assays are between 1:500 and 1:5000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS, pH 7.2. No preservative added.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C
Short term: +4°C. Avoid repeat freeze-thaw cycles.

Details zu Insulin

Target: Insulin (INS)

Andere Bezeichnung: Insulin

Hintergrund: Name/Gene ID: INS
Family: Hormone

Synonyms: INS, IRDN, IDDM2, Preproinsulin, ILPR, Insulin, MODY10, Proinsulin

Gen-ID: 3630

Pathways: NF-kappaB Signalweg, RTK Signalweg, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagie, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation