eIF4EBP1 Antikörper (truncated)

Produktnummer ABIN2464007

Der Maus Monoklonal anti-eIF4EBP1 Antikörper wird verwendet zum Nachweis von eIF4EBP1 in Proben von Human. Er wurde validiert für IHC.

Kurzübersicht für eIF4EBP1 Antikörper (truncated) (ABIN2464007)

Target: eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser eIF4EBP1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: 11G12C11

Produktdetails anti-eIF4EBP1 Antikörper

Bindungsspezifität: truncated

Aufreinigung: Antibody is purified by protein A affinity chromatography.

Immunogen: Ni-NTA purified truncated recombinant 4EBP1 expressed in E. Coli strain BL21 (DE3).

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: IHC:1:500 - 1:1,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Purified antibody in PBS containing 0.03 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Lagerung: -20 °C

Informationen zur Lagerung: 4E-BP1 monoclonal antibody can be stored at -20 °C, stable for one year.

Details zu eIF4EBP1

Target: eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))

Andere Bezeichnung: 4E-BP1

Hintergrund: 4E-BP1(eukaryotic translation Initiation Factor 4E Binding Protein 1),also called ELF4EBP1/BP-1/PHAS-I ,which is located on chromosome 8p12, with 118-amino acid protein (about 13 kDa). Binding of eIF4EBP1 to eIF4E is reversible and is dependent on the phosphorylation status of eIF4EBP1. Non phosphorylated eIF4EBP1 will bind strongly to eIF4E while(24 kDa), the phosphorylated form will not. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating eIF4EBP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the eIF4EBP1-eIF4E interaction.

Gen-ID: 1978

UniProt: Q13541

Pathways: MAPK Signalweg, PI3K-Akt Signalweg, RTK Signalweg, AMPK Signaling, Regulation of Cell Size, BCR Signaling