HRAS Antikörper

Produktnummer ABIN238418

Der Maus Monoklonal Anti-HRAS-Antikörper wurde für WB und EIA validiert. Er ist geeignet, HRAS in Proben von Human zu detektieren.

Kurzübersicht für HRAS Antikörper (ABIN238418)

Target: HRAS (HRas proto-oncogene, GTPase (HRAS))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HRAS Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Klon: H-Ras-03

Produktdetails anti-HRAS Antikörper

Spezifität: The antibody H-Ras-03 reacts with human H-Ras, an ubiquitously expressed 21 kDa intracellular protein. Although reactivity with other species has not been determined, it is probable as the epitope is highly conserved among animals.

Produktmerkmale: Synonyms: GTPase HRas, HRAS1, p21ras, H-Ras-1, c-H-ras, Ha-Ras, H-Ras, v-Ha-ras Harvey rat sarcomaviral oncogene homolog

Aufreinigung: Protein A affinity chromatography.

Immunogen: Peptide corresponding to amino acids DIHQYREQIKRVKDSDDC of human H-Ras protein.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA. Western Blotting under Reducing conditions (0.5-1 μg/mL): Positive control: RAJI human Burkitt lymphoma cell lineHeLa human cervix carcinoma cell lineK567 human leukemia cell lineSample preparation: Resuspend approx.50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysatewith reducing Laemmli SDS-PAGE sample buffer. Boil for 5 min.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH ~7.4, 15 mM Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid freeze/thaw cycles.
Shelf life: One year from despatch.

Haltbarkeit: 12 months

Details zu HRAS

Target: HRAS (HRas proto-oncogene, GTPase (HRAS))

Andere Bezeichnung: HRAS

Hintergrund: H-Ras is one of three ubiquitously isoforms of Ras GTPase that operate at the intracellular leaflet of the plasma membrane to regulate multiple signal transduction pathways, such as mitogen-activated protein kinase (MAPK) cascade. H Ras is anchored to the plasma membrane by farnesyl and two palmityl residues. GTP loading decreases H-Ras affinity for lipid rafts and allows the protein to target to nonraft microdomains, the primary sites of H-Ras signaling. Sos protein and other guanine nucleotide-exchange factors catalyze dissociation of GDP from Ras. Besides its roles in the plasma membrane, active H-Ras also diffuses through the cytoplasm on nanoparticles termed rasosomes, which is dependent on Ras palmitoylation.Synonyms: GTPase HRas, H-Ras, H-Ras-1, HRAS1, Ha-Ras, c-H-ras, p21ras, v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Gen-ID: 3265

UniProt: P01112

Pathways: p53 Signalweg, MAPK Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Hepatitis C, Autophagie, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling, BCR Signaling