HRAS Antikörper (AA 62-76)

Produktnummer ABIN224929

Produktdetails anti-HRAS Antikörper

Target: HRAS (HRas proto-oncogene, GTPase (HRAS))

Bindungsspezifität: AA 62-76

Reaktivität: Human, Maus, Ratte

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser HRAS Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Neutralization (Neut)

Spezifität: Recognizes phosphorylated and non-phosphorylated human, rat and mouse ras. Does not cross-react with the rap protein. Neutralizes the biological and biochemical activities by binding to residues E63, S65, A66, M67, Q70 and R73. Positive Controls: SW480 or Y1 cells (IF and IP), normal skin (IHC, frozen and paraffin), A431, Hela or ML-1 cells (WB).

Aufreinigung: Purified

Immunogen: Recombinant p21 ras protein. Epitope: aa62-76 of human ras.

Type of Immunogen: Recombinant protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Approved: IF (2.5 μg/mL), IHC, IHC-Fr, IHC-P, IP, Neut, WB (2.5 μg/mL)

Usage: Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry and Neutralization. Immunofluorescence: 2.5 μg/mL. Western Blot (chemiluminescence detection): 2.5 μg/mL. Immunohistochemistry (Frozen): 5 μg/mL. Immunohistochemistry: 1-5 μg/mL. Staining of formalin fixed, paraffin embedded tissue requires pepsin pretreatment. Use saponin to permeablize. Immunoprecipitation (autoradiographic detection): 1 μg/sample SW480 or Y1 cells. Use rabbit anti-rat IgG plus Protein A or goat anti-rat IgG plus Protein G. Protein A alone will not immunoprecipitate rat IgG. Doublets corresponding to farnesylated- and non-farnesylated ras may be observed. Detects both phosphorylated and non-phosphorylated human ras as well as the p21 translational products of the H-, K- and N-ras human genes.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu HRAS

Target: HRAS (HRas proto-oncogene, GTPase (HRAS))

Andere Bezeichnung: HRAS / H-Ras (HRAS Produkte)

Synonyme: C-BAS/HAS antikoerper, C-H-RAS antikoerper, C-HA-RAS1 antikoerper, CTLO antikoerper, H-RASIDX antikoerper, HAMSV antikoerper, HRAS1 antikoerper, K-RAS antikoerper, N-RAS antikoerper, RASH1 antikoerper, hras antikoerper, zgc:110250 antikoerper, HRAS antikoerper, H-RAS antikoerper, c-H-ras antikoerper, H-Ras antikoerper, K-Ras antikoerper, hras1 antikoerper, rash1 antikoerper, ras antikoerper, N-Ras antikoerper, c-bas/has antikoerper, H-ras antikoerper, Ha-ras antikoerper, Harvey-ras antikoerper, Hras-1 antikoerper, Kras2 antikoerper, c-Ha-ras antikoerper, c-rasHa antikoerper, hrasl antikoerper, zgc:110734 antikoerper, HRas proto-oncogene, GTPase antikoerper, v-Ha-ras Harvey rat sarcoma viral oncogene homolog a antikoerper, neuroblastoma RAS viral (v-ras) oncogene homolog pseudogene antikoerper, Harvey rat sarcoma viral oncogene homolog L homeolog antikoerper, Harvey rat sarcoma viral oncogene homolog antikoerper, Harvey rat sarcoma virus oncogene antikoerper, NRAS proto-oncogene, GTPase antikoerper, -Ha-ras Harvey rat sarcoma viral oncogene homolog b antikoerper, HRAS antikoerper, hrasa antikoerper, LOC733587 antikoerper, Hras antikoerper, hras.L antikoerper, hras antikoerper, NRAS antikoerper, hrasb antikoerper

Hintergrund: Name/Gene ID: HRAS
Subfamily: RAS oncogene
Family: Ras GTPase superfamily IPR001806

Synonyms: HRAS, C-H-RAS, C-has/bas p21 protein, C-ras-Ki-2 activated oncogene, C-HA-RAS1, CTLO, H-ras, H-RASIDX, GTP- and GDP-binding peptide B, GTPase HRas, H-Ras-1, HAMSV, HRAS1, K-RAS, Ha-Ras, Ha-Ras1 proto-oncoprotein, Transforming protein p21, N-ras, p21ras, RASH1, C-BAS/HAS, C-ha-ras, p19 H-RasIDX protein

Gen-ID: 3265

UniProt: P01112

Pathways: p53 Signalweg, MAPK Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Hepatitis C, Autophagie, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling, BCR Signaling