ERK1 Antikörper (AA 372-406)

Produktnummer ABIN199418

Produktdetails anti-ERK1 Antikörper

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Bindungsspezifität: AA 372-406

Reaktivität: Maus, Ratte, Kaninchen, Hamster

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ERK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Functional Studies (Func)

Spezifität: Recognizes rat MAPK1/Erk1 at 44kD and MAPK2/Erk2 at 42kD. Species cross-reactivity: Human, mouse, chicken and starfish. Species sequence Homology: Chinese hamster: 35/35, mouse: 34/35, human: 32/35.

Homologie: Percent identity by BLAST analysis: Marmoset, Mouse, Rat, Hamster, Panda, Rabbit, Opossum (100%) Monkey, Bovine, Dog, Bat, Horse, Platypus (97%) Human, Gorilla, Gibbon, Elephant (94%) Orangutan, Pig, Turkey, Chicken, Pufferfish, Zebrafish (87%) Xenopus (84%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide (CGG-PFTFDMELDDLPKERLKELIFQETARFQPGAPEAP) corresponding to the C-terminal 35 amino acids of rat 44kD MAP Kinase 2/ Erk2 (KLH coupled). Percent identity by BLAST analysis: Marmoset, Mouse, Rat, Hamster, Panda, Rabbit, Opossum (100%), Monkey, Bovine, Dog, Bat, Horse, Platypus (97%), Human, Gorilla, Gibbon, Elephant (94%), Orangutan, Pig, Turkey, Chicken, Pufferfish, Zebrafish (87%), Xenopus (84%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: Func, IP, WB (0.1 - 2 μg/mL)

Usage: Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 0.1-2 μg/mL detects MAP kinases in RIPA lysates of mouse 3T3/A31 fibroblasts and L6 cells. 3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-MAP Kinase 1/2 (0.1 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation Kinase Assay: 4 μg immunoprecipitates active MAP kinases from a lysate of NGF-stimulated (50 ng/mL) PC-12 cells, as demonstrated using the MAPK Immunoprecipitation Kinase Cascade Kit.

Kommentare:

Target Species of Antibody: Rat

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.2 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 0.1 mM EDTA.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu ERK1

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Andere Bezeichnung: MAPK3 / ERK1 (MAPK3 Produkte)

Synonyme: ERK-1 antikoerper, ERK1 antikoerper, ERT2 antikoerper, HS44KDAP antikoerper, HUMKER1A antikoerper, P44ERK1 antikoerper, P44MAPK antikoerper, PRKM3 antikoerper, p44-ERK1 antikoerper, p44-MAPK antikoerper, Erk-1 antikoerper, Erk1 antikoerper, Ert2 antikoerper, Esrk1 antikoerper, Mnk1 antikoerper, Mtap2k antikoerper, Prkm3 antikoerper, p44 antikoerper, p44erk1 antikoerper, p44mapk antikoerper, ERK3 antikoerper, ERK6 antikoerper, P38GAMMA antikoerper, PRKM12 antikoerper, SAPK-3 antikoerper, SAPK3 antikoerper, fi06b09 antikoerper, wu:fi06b09 antikoerper, zERK1 antikoerper, Tb08.10J17.940 antikoerper, MAPK1 antikoerper, MNK1 antikoerper, AW123708 antikoerper, Erk6 antikoerper, P38gamma antikoerper, Prkm12 antikoerper, Sapk3 antikoerper, ATMAPK3 antikoerper, ATMPK3 antikoerper, T6D9.4 antikoerper, mitogen-activated protein kinase 3 antikoerper, mitogen-activated protein kinase 3 antikoerper, mitogen-activated protein kinase 12 antikoerper, mitogen activated protein kinase 3 antikoerper, mitogen-activated serine/threonine-protein kinase antikoerper, MAPK3 antikoerper, Mapk3 antikoerper, MAPK12 antikoerper, mapk3 antikoerper, Tc00.1047053509475.10 antikoerper, Tb927.8.3550 antikoerper, Mapk12 antikoerper, CEK1 antikoerper, MPK3 antikoerper

Hintergrund: Name/Gene ID: MAPK3
Subfamily: MAPK
Family: Protein Kinase

Synonyms: MAPK3, ERK-1, ERT2, HUMKER1A, HS44KDAP, MAP kinase 1, MAP kinase isoform p44, MAPK 1, MAPK 3, p44, p44MAPK, p44-MAPK, p44ERK1, Insulin-stimulated MAP2 kinase, MAP kinase 3, p44-ERK1, ERK1, PRKM3

Gen-ID: 5595

UniProt: P27361

Pathways: MAPK Signalweg, RTK Signalweg, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteine