MAPK3 Antikörper (Mitogen-Activated Protein Kinase 3) (Atto 594)

Details for Product anti-MAPK3 Antibody No. ABIN2486607
Target
Reaktivität
Huhn, Rind (Kuh), Drosophila melanogaster, Human, Maus, Ratte, Schaf, Xenopus laevis
355
230
220
50
45
42
42
26
19
12
11
8
7
6
5
5
5
3
3
2
2
1
1
1
1
1
Wirt
Kaninchen
299
67
8
5
1
Klonalität
Polyklonal
Konjugat
Dieser MAPK3 Antikörper ist konjugiert mit Atto 594
31
25
23
15
14
14
7
7
7
7
7
7
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
Applikation
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
300
149
135
71
54
41
40
39
36
29
19
14
7
6
3
2
1
1
Optionen
Immunogen A 35 residue synthetic peptide, corresponding to Erk1 MAP kinase with the CGG spacer group added and the peptide coupled to KLH.
Spezifität Detects ~44kda (ERK1) and ~42 kDa (ERK2).
Reinigung Peptide Affinity Purified
Target
Andere Bezeichnung ERK1 (MAPK3 Antibody Abstract)
Hintergrund The extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2), also called p44 and p42 MAP kinases, are members of the Mitogen Activated Protein Kinase (MAPK) family of proteins found in all eukaryotes. Because the 44 kDa ERK1 and the 42 kDa ERK2 are highly homologous and both function in the same protein kinase cascade, the two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase (1). They are both located in the cytosol and mitochondria (2). While the role of cytosol ERK1/2 is well studied and involved in multiple cellular functions (2), the role of mitochondrial ERK1/2 remains poorly understood. Both ERK 1 and 2 are activated by MEK1 or MEK2, by dual phosphorylation of a threonine and tyrosine residue in the activation loop (TEY motif) (1, 3). Either phosphorylation alone can induce an electrophoretic mobility shift, but both are required for activation of the kinase. This dual phosphorylation is efficiently detected by phosphorylation state-specific antibody directed to the pTEpY motif. Once activated, MAP kinases phosphorylate a broad spectrum of substrates, including cytoskeletal proteins, translation regulators, transcription factors, and the Rsk family of protein kinases (4). ERK1/2 activation is generally thought to confer a survival advantage to cells (5), however there is increasing evidence that suggests that the activation of ERK1/2 also contributes to cell death under certain conditions (5). ERK1/2 also is activated in neuronal and renal epithelial cells upon exposure to oxidative stress and toxicants or deprivation of growth factors, and inhibition of the ERK pathway blocks apoptosis (5).
Gen-ID 50689
NCBI Accession NP_059043
UniProt P21708
Pathways MAPK Signalweg, RTK Signalweg, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals
Applikations-hinweise
  • WB (1:1000)
  • IHC (1:100)
  • ICC/IF (1:100)
  • FCM (1:100)
  • optimal dilutions for assays should be determined by the user.
Kommentare

A 1:1000 dilution of ABIN2486607 was sufficient for detection of ERK1/2 in 20 μg of HeLa cell lysate by ECL immunoblot analysis.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Lagerung -20 °C
Bilder
Image no. 1 for anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody (Atto 594) (ABIN2486607) Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Erk1/2 Polyclonal A...
Image no. 2 for anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody (Atto 594) (ABIN2486607) Western blot analysis of Human Cell line lysates showing detection of ERK1 protein us...
Image no. 3 for anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody (Atto 594) (ABIN2486607) Immunohistochemistry analysis using Rabbit Anti-ERK1 Polyclonal Antibody . Tissue: In...
Image no. 4 for anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody (Atto 594) (ABIN2486607) Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ERK1 Polyclonal Ant...
Image no. 5 for anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody (Atto 594) (ABIN2486607) Immunohistochemistry analysis using Rabbit Anti-ERK1 Polyclonal Antibody . Tissue: ba...
Image no. 6 for anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody (Atto 594) (ABIN2486607) Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Erk1/2 Polyclonal A...