p53 Antikörper (pSer315)
Kurzübersicht für p53 Antikörper (pSer315) (ABIN196788)
Target
Alle p53 (TP53) Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
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Bindungsspezifität
- pSer315
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Spezifität
- This antibody detects endogenous levels of p53 only when phosphorylated at Serine 315.
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Aufreinigung
- Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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Immunogen
- The antiserum was produced against synthesized phosphopeptide derived from human p53 around the phosphorylation site of Serine 315 (S-S-SP-P-Q).
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Applikationshinweise
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Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin Sections: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Konzentration
- 1.0 mg/mL
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Buffer
- PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handhabung
- Avoid repeated freezing and thawing.
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Lagerung
- -20 °C
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Informationen zur Lagerung
- Store the antibody (in aliquots) at -20 °C.
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- p53 (TP53) (Tumor Protein P53 (TP53))
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Andere Bezeichnung
- p53 (TP53)
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Hintergrund
- Synonyms: Cellular tumor antigen p53, NY-CO-13, Phosphoprotein p53, Tumor suppressor p53
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Gen-ID
- 7157
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NCBI Accession
- NP_000537
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UniProt
- P04637
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Pathways
- p53 Signalweg, MAPK Signalweg, PI3K-Akt Signalweg, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagie, Warburg Effekt
Target
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