p53 Antikörper (AA 364-393)

Produktnummer ABIN1882106

Der Kaninchen Polyklonal Anti-p53-Antikörper wurde für WB und IHC (p) validiert. Er ist geeignet, p53 in Proben von Human zu detektieren. Es sind 5+ Publikationen verfügbar.

Kurzübersicht für p53 Antikörper (AA 364-393) (ABIN1882106)

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser p53 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: RB2571

Produktdetails anti-p53 Antikörper

Bindungsspezifität: AA 364-393

Aufreinigung: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This p53 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 364-393 amino acids from human p53.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:1000. WB: 1:2000. IHC-P: 1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Haltbarkeit: 6 months

Referenzen für anti-p53 Antikörper (ABIN1882106)

Chen, Chen: "MDM2-ARF complex regulates p53 sumoylation." in: Oncogene, Vol. 22, Issue 34, pp. 5348-57, (2003) (PubMed).

Melchior, Hengst: "SUMO-1 and p53." in: Cell cycle (Georgetown, Tex.), Vol. 1, Issue 4, pp. 245-9, (2002) (PubMed).

Muller, Berger, Lehembre, Seeler, Haupt, Dejean: "c-Jun and p53 activity is modulated by SUMO-1 modification." in: The Journal of biological chemistry, Vol. 275, Issue 18, pp. 13321-9, (2000) (PubMed).

Gostissa, Hengstermann, Fogal, Sandy, Schwarz, Scheffner, Del Sal: "Activation of p53 by conjugation to the ubiquitin-like protein SUMO-1." in: The EMBO journal, Vol. 18, Issue 22, pp. 6462-71, (2000) (PubMed).

Rodriguez, Desterro, Lain, Midgley, Lane, Hay: "SUMO-1 modification activates the transcriptional response of p53." in: The EMBO journal, Vol. 18, Issue 22, pp. 6455-61, (2000) (PubMed).

Details zu p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Andere Bezeichnung: p53

Hintergrund: Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. P53 is subject to modification by conjugation of SUMO-1. A p53 mutant deficient for MDM2 binding is poorly sumoylated in vivo compared to wild-type p53. Overexpression of MDM2 increases the level of p53 sumoylation, which is further stimulated by expression of ARF. These results show that p53 sumoylation is regulated by MDM2- and ARF-mediated nucleolar targeting.

Molekulargewicht: 43653

NCBI Accession: NP_000537, NP_001119584, NP_001119585, NP_001119586, NP_001119587, NP_001119588, NP_001119589, NP_001119590, NP_001263624, NP_001263625, NP_001263626, NP_001263627, NP_001263628, NP_00126

UniProt: P04637

Pathways: p53 Signalweg, MAPK Signalweg, PI3K-Akt Signalweg, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagie, Warburg Effekt