RIPK1 Antikörper

Produktnummer ABIN127073

Der Kaninchen Polyklonal anti-RIPK1 Antikörper wird verwendet zum Nachweis von RIPK1 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IP, IHC (p) und IHC (fro).

Kurzübersicht für RIPK1 Antikörper (ABIN127073)

Target: RIPK1 (Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RIPK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-RIPK1 Antikörper

Spezifität: The antibody detects 74 kDa RIP in Western blot analysis.

Kreuzreaktivität (Details): Species reactivity (tested):Human, Mouse and Rat.

Aufreinigung: Affinity Chromatography.

Immunogen: Synthetic peptide surrounding amino acid 645 of Human RIP

Anwendungsinformationen

Applikationshinweise: Western Blot: 2-6 μg/mL. Immunoprecipitation. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: PBS, pH 7.2, containing 30 % Glycerol, 0.5 % BSA as stabilizer and 0.01 % Thimerosal as preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: Store the antibody undiluted at -20 °C or (in aliquots) at -70 °C for long term storage.

Details zu RIPK1

Target: RIPK1 (Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1))

Andere Bezeichnung: RIP

Hintergrund: RIP (Receptor Interacting Protein) is a 74 kDa Ser/Thr kinase which interacts with CD95 (Fas/Apo1) receptor and the tumor necrosis factor receptor (TNFR1). It is a cell death domain adapter protein which can bind to the adapter proteins TRADD, RAID, and TRAF2. RIP contains an N-terminal region with homology to protein kinase, an intermediate domain capable of association with MAPKKK and a C-terminal region containing an intracellular death domain motif. RIP activates both p38 MAP kinase and SAPK families. In vitro, RIP induces apoptosis, as well as SAPK/JNK and NF-kB activation. RIP possesses kinase activity as it autophosphorylates itself on serine and threonine residues.Synonyms: Cell death protein RIP, RIPK1, Receptor-Interacting Protein, Receptor-interacting serine/threonine-protein kinase 1, Serine/threonine-protein kinase RIP

Gen-ID: 8737

NCBI Accession: NP_003795

UniProt: Q13546

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Activation of Innate immune Response, Inositol Metabolic Process, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Negative Regulation of intrinsic apoptotic Signaling, SARS-CoV-2 Protein Interaktom, Ubiquitin Proteasome Pathway