MAPK10 Antikörper

Produktnummer ABIN126824

Der Maus Monoklonal Anti-MAPK10-Antikörper wurde für WB validiert. Er ist geeignet, MAPK10 in Proben von Human zu detektieren.

Kurzübersicht für MAPK10 Antikörper (ABIN126824)

Target: MAPK10 (Mitogen-Activated Protein Kinase 10 (MAPK10))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MAPK10 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Klon: 4G6

Produktdetails anti-MAPK10 Antikörper

Spezifität: Antibody AM00080PU-N specifically interacts with the N-terminus of SAPK1 beta/jnk3 kinase. Shows no cross-reactivity with jnk1 or jnk2

Produktmerkmale: Synonyms: Stress-activated protein kinase JNK3, c-Jun N-terminal kinase 3, JNK3A, PRKM10, MAPkinase p49 3F12, Mitogen-activated protein kinase 10

Aufreinigung: Purified from serum-free cell culture supernatant by subsequent ultrafiltration and size exclusion chromatography

Bestandteile: incl. positive Control

Immunogen: Peptide conjugated to hemocyaninEpitope: N-terminusRemarks: Molecular weight of antigen 54 kDa

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended buffer: Casein/Tween 20 based blocking and blot incubation buffer. Use cell lysate from untreated SH-SY5Y cells delivered with this product as positive control: see protocol below!
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: Positive Control Cell Lysate: Untreated SH-SY5YFormat: Lyophilized cell lysate from serum starved SH-SY5Y cells. Reconstitute by addition of 200 µl H20. After complete solubilization add 200 µl 2xSDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Applications: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20. 000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended forapplications with native proteins such as immunoprecipitation.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 2 x PBS / 0.09 % Na-azide / PEG and Sucrose

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -20 °C to -80 °C. Thaw aliquots at 37 °C. Thawed aliquots may be stored at2-8 °C up to 3 months. Avoid repeated freeze / thaw cycles.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu MAPK10

Target: MAPK10 (Mitogen-Activated Protein Kinase 10 (MAPK10))

Andere Bezeichnung: MAPK10

Hintergrund: Stress-activated Protein Kinases (SAPKs) are strongly activated in response to adverse stimuli such as heat and osmotic shock, UV light and other DNA-damaging reagents, and inhibitors of protein synthesis. They are also activated strongly in response to agonists that are released or produced under conditions of stress, such as proinflammatory cytokines. The SAPK1/jnk family consists of 3 isoforms: SAPK1alpha/jnk2, SAPK1beta/jnk3, SAPK1gamma/jnk1.Synonyms: JNK3A, MAP kinase p49 3F12, Mitogen-activated protein kinase 10, PRKM10, Stress-activated protein kinase JNK3, c-Jun N-terminal kinase 3

Gen-ID: 5602

UniProt: P53779

Pathways: MAPK Signalweg, WNT Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades