p53 Antikörper (AA 181-190)

Produktnummer ABIN120058

Dieser Maus Monoklonal Antikörper detektiert spezifisch p53 in WB, IHC (p), IP und IHC (fro). Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für p53 Antikörper (AA 181-190) (ABIN120058)

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser p53 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: DO-11

Produktdetails anti-p53 Antikörper

Bindungsspezifität: AA 181-190

Spezifität: This antibody recognises the p53 tumour suppressor protein, binding to both wild type and mutant forms. DO-11 recognises an epitope within the central region of p53 between amino acids 181 - 190.

Aufreinigung: Affinity chromatography on Protein G

Immunogen: Recombinant human p53. Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63Ag8.653 myeloma cell line.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/1000. Immunoprecipitation. Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections: 1/1000, This product does require antigen retrieval using heat treatment prior to staining of paraffin sections, Sodium citrate buffer pH 6.0 is recommended for this purpose. Recommended Positive Control: Colon or breast carcinoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Andere Bezeichnung: p53 (TP53)

Hintergrund: Synonyms: Cellular tumor antigen p53, NY-CO-13, Phosphoprotein p53, Tumor suppressor p53

Gen-ID: 7157

NCBI Accession: NP_000537

UniProt: P04637

Pathways: p53 Signalweg, MAPK Signalweg, PI3K-Akt Signalweg, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagie, Warburg Effekt