p53 Antikörper (AA 20-25) (Biotin)

Produktnummer ABIN120052

Dieser Maus Monoklonal Antikörper detektiert spezifisch p53 in FACS. Es zeigt Reaktivität gegenüber Proben von Human, Rind (Kuh), Pferd und Cat.

Kurzübersicht für p53 Antikörper (AA 20-25) (Biotin) (ABIN120052)

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Reaktivität: Human, Rind (Kuh), Pferd, Cat

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser p53 Antikörper ist konjugiert mit Biotin

Applikation: Flow Cytometry (FACS)

Klon: DO-1

Produktdetails anti-p53 Antikörper (Biotin)

Bindungsspezifität: AA 20-25

Spezifität: This antibody recognizes the p53 tumour suppressor protein, binding to both wild type and mutant forms. Clone DO-1 binds to both wild type and mutant forms of the p53 protein found in various malignancies (Kern et al. 1992). p53 is important in multicellular organisms, where it regulates cell cycle progression to allow DNA repair or apoptosis in the case of irreparably damaged cells (Haupt et al. 2003) and thus functions as a tumor suppressor that is involved in preventing cancer. Mutations in the p53 gene are found in about half the cases of human cancer (Joerger and Fersht 2007). DO-1 recognizes an epitope at the N-terminal end of p53 between amino acids 20-25, common to isoforms 1-3 of p53.

Kreuzreaktivität (Details): Species reactivity (tested):Human, Horse, Bovine, Cat, Green Monkey and Sheep.

Aufreinigung: Affinity chromatography on Protein G

Immunogen: Recombinant human p53. Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63Ag8.653 myeloma cell line.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide, 1 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Andere Bezeichnung: p53 (TP53)

Hintergrund: Synonyms: Cellular tumor antigen p53, NY-CO-13, Phosphoprotein p53, Tumor suppressor p53

Gen-ID: 7157

NCBI Accession: NP_000537

UniProt: P04637

Pathways: p53 Signalweg, MAPK Signalweg, PI3K-Akt Signalweg, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagie, Warburg Effekt