IL12 Antikörper

Produktnummer ABIN1177291

Dieses Ratte Monoklonal-Antikörper erkennt spezifisch IL12 in ELISA, WB, Neut und IP. Er zeigt eine Reaktivität gegenüber Maus und wurde in 4+ Publikationen erwähnt.

Kurzübersicht für IL12 Antikörper (ABIN1177291)

Target: IL12 (Interleukin 12 (IL12))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser IL12 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Neutralization (Neut), Immunoprecipitation (IP)

Klon: C17-8

Produktdetails anti-IL12 Antikörper

Marke: BD Pharmingen™

Produktmerkmale: Neutralization Activity: This antibody has been reported to be useful for the neturalization of recombinant mouse IL-12 (p70). Neutralization activity may be measured with a proliferation assay using 300 pg/mL recombinant mouse IL-12 (p70) (Cat. No. 554592) to stimulate 2D6 cells at 1x10^5 cells/mL as indicator cells (i.e. preincubation of the antibody with recombinant mouse IL-12 (p70) for 60 minutes).
50% Neutralization (ND50) at 4 - 100 ng/mL
>95% Neutralization at 60 - 600 ng/mL

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Recombinant Mouse IL-12 p70

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: ELISA (IL-12 p70 Detection): The biotinylated C17.8 antibody (Cat. No. 554476) is useful as a detection antibody for a sandwich ELISA for specifically measuring mouse IL-12 p70 protein levels. Biotinylated C17.8 antibody can be paired with the purified 9A5 (Cat. No. 554658) as the capture antibody and with recombinant mouse IL-12 p70 (Cat. No.554592) as the standard. For testing mouse IL-12 p70 in complex biological fluids, such as serum or plasma, investigators may wish to consider using the Mouse IL-12 (p70) BD OptEIA™ ELISA Set (Cat. No. 555256).
ELISA (IL-12 p40 Detection): The biotinylated C17.8 antibody (Cat. No. 554476) is useful as a detection antibody for a sandwich ELISA measuring IL-12 p40 protein levels (free p40 monomer as well as p40 complexes as homodimer or heterodimer). Biotinylated C17.8 antibody can be paired with the purified C15.6 antibody (Cat. No. 551219) as the capture antibody, and with recombinant mouse IL-12 p70 protein (Cat. No.554592) or IL-12 p40 protein (Cat. No. 554594) as the standard. For testing mouse IL-12 p40 in complex biological fluids, such as serum or plasma, investigators may wish to consider using the Mouse IL-12 (p40) BD OptEIA™ ELISA Set (Cat. No. 555165).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Konservierungsmittel: Azide free

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Referenzen für anti-IL12 Antikörper (ABIN1177291)

Maruo, Ahn, Yu, Tomura, Wysocka, Yamamoto, Kobayashi, Hamaoka, Trinchieri, Fuijiwara: "Establishment of an IL-12-responsive T cell clone: its characterization and utilization in the quantitation of IL-12 activity." in: Journal of leukocyte biology, Vol. 61, Issue 3, pp. 346-52, (1997) (PubMed).

Neurath, Fuss, Kelsall, Stüber, Strober: "Antibodies to interleukin 12 abrogate established experimental colitis in mice." in: The Journal of experimental medicine, Vol. 182, Issue 5, pp. 1281-90, (1995) (PubMed).

Wysocka, Kubin, Vieira, Ozmen, Garotta, Scott, Trinchieri: "Interleukin-12 is required for interferon-gamma production and lethality in lipopolysaccharide-induced shock in mice." in: European journal of immunology, Vol. 25, Issue 3, pp. 672-6, (1995) (PubMed).

DAndrea, Rengaraju, Valiante, Chehimi, Kubin, Aste, Chan, Kobayashi, Young, Nickbarg: "Production of natural killer cell stimulatory factor (interleukin 12) by peripheral blood mononuclear cells." in: The Journal of experimental medicine, Vol. 176, Issue 5, pp. 1387-98, (1992) (PubMed).

Details zu IL12

Target: IL12 (Interleukin 12 (IL12))

Andere Bezeichnung: IL-12 (p40/p70)

Hintergrund: The C17.8 antibody reacts with the free p40 monomer of IL-12 as well as the p40 subunit complexed as homodimer or complexed with p35 as a p70 heterodimer. Clone C17.8 has also been shown to be cross-reactive with mouse IL-23, which also contains the p40 subunit. The immunogen used to generate C17.8 hybridoma was recombinant mouse IL-12 p70.

Pathways: JAK-STAT Signalweg, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome