IL-1 beta Antikörper

Produktnummer ABIN115866

Der Kaninchen Polyklonal anti-IL-1 beta Antikörper (ABIN115866) detektiert spezifisch IL-1 beta in WB, EIA und Func. Dieser Antikörper reagiert spezifisch mit Proben aus Maus.

Kurzübersicht für IL-1 beta Antikörper (ABIN115866)

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IL-1 beta Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Produktdetails anti-IL-1 beta Antikörper

Spezifität: This antibody detects Murine IL-1 beta

Aufreinigung: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant mIL-1 beta.

Anwendungsinformationen

Applikationshinweise: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mIL-1 beta (10pg/mL), a concentration of 0.57-0.86 μg/mL of this antibody is required. Sandwich ELISA: To detect Mouse IL-1 beta by Sandwich ELISA (using 100 μL/well antibody solution) aconcentration of at least 0.5-2.0 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with Biotinylated anti-Murine IL-1 beta antibody(PP006B1/PP006B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-1 beta. Western Blot: To detect mIL-1 beta by Western Blot analysis this antibody can be used at a concentrationof 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant mIL-1 beta is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Details zu IL-1 beta

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Andere Bezeichnung: Interleukin-1 beta / IL-1B

Hintergrund: Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types, and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin1 alpha (IL1 alpha) and interleukin1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25 % homology in their amino acid sequence.Synonyms: Catabolin, IL-1 beta, IL1 beta, IL1B, IL1F2

Gen-ID: 16176

NCBI Accession: NP_032387

UniProt: P10749

Pathways: NF-kappaB Signalweg, Interferon-gamma Pathway, TLR Signalweg, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagie, Cancer Immune Checkpoints, Inflammasome