CD40 Antikörper

Produktnummer ABIN111321

Dieses Anti-CD40-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CD40 in FACS, Func, IHC (p) und IHC (fro). Geeignet für Human.

Kurzübersicht für CD40 Antikörper (ABIN111321)

Target: CD40

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD40 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: MAB89

Produktdetails anti-CD40 Antikörper

Spezifität: CD40 B cells.

Immunogen: Anti-mu activated tonsillar human B cells.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Fluorescent Microscopy or Flow Cytometry: 2 μg/5x10e5 cells/test. Activation assays: Working concentration: 10 ng/mL. Study of of B-cell proliferation, B-cell growth and activation factors. Functional Study of B-cell subpopulations.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1 mL of distilled water.

Konzentration: 0.2 mg/mL (prior to lyophilization)

Buffer: PBS buffer containing 1 mg/mL BSA as stabilizer without preservatives.

Konservierungsmittel: Without preservative

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2 - 8 °C.

Details zu CD40

Target: CD40

Andere Bezeichnung: CD40

Hintergrund: CD40 is a 49 kDa type I integral membrane protein of the NGF receptor family. CD40 is found on normal and neoplastic B cells.(1) It is a pan-B marker, absent only from terminally differentiated plasma cells. CD40 is predicted to function in the process of B cell selection in the germinal center. Studies demonstrated that it induces strong homotypic adhesions in resting B cells and, together with Interleukin-4 (IL-4) maintains the cell cycle of B blasts and promotes the switch to IgE secretion. (1,2) Purified B cells cultured in the CD40 system in the presence of IL-10 differentiate into plasma cells and secrete large amounts of immunoglobulins. (3) The ligand for CD40 is a membrane glycoprotein on activated T cells that induces B cell proliferation and immunoglobulin secretion. (4) This clone acts in synergy with IL-4 to induce the increase of B-cell volume and the proliferation of anti IgM antibody-co-stimulated or pre-activated B cells. (5)Synonyms: B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40, TNFRSF5, Tumor necrosis factor receptor superfamily member 5

Gen-ID: 958

NCBI Accession: NP_001241

UniProt: P25942

Pathways: NF-kappaB Signalweg, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints