CD40 Antikörper (AA 29-107)

Produktnummer ABIN6941187

Dieser Maus Monoklonal Antikörper detektiert spezifisch CD40 in FACS, WB, ELISA und IHC. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für CD40 Antikörper (AA 29-107) (ABIN6941187)

Target: CD40

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD40 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Klon: C40-2383

Produktdetails anti-CD40 Antikörper

Bindungsspezifität: AA 29-107

Verwendungszweck: CD40 / TNFRSF5 / CD40L-Receptor Antibody

Spezifität: CD40 is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. CD40 is expressed on B-lymphocytes, follicular dendritic cells, bone marrow-derived dendritic cells, thymic epithelium, and interdigitating cells in the T-cell zones of secondary lymphoid organs.

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment of human CD40 protein (around aa 29-107) (exact sequence is proprietary)

Isotyp: IgG2b, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: U2OS, L-428 cells. Tonsil, Spleen or Thymus.

Known Application: ELISA (For coating, order antibody without BSA),Flow Cytometry (1-2 μg/million cells),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu CD40

Target: CD40

Andere Bezeichnung: CD40

Hintergrund:

B-cell surface antigen CD40, Bp50, CD40, CD40L receptor, GP39, HIGM1, IGM, IMD3, p50, TBAM, TNF receptor superfamily member 5, TNFRSF5, TRAP

spleen or thymus.,Raji, U2OS or L-428 cells. Human tonsil

Molekulargewicht: 43kDa

Gen-ID: 958, 472860

UniProt: P25942

Pathways: NF-kappaB Signalweg, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints