anti-Maus FZD1 Antikörper für Immunohistochemistry (Paraffin-embedded Sections)

Recommended FZD1 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Frizzled Family Receptor 1 (Fzd1) Antikörper
  • CG17697
  • CG3646
  • DFZ1
  • DFz
  • DFz1
  • Dfz1
  • Dm Fz1
  • Dmel\\CG17697
  • FZ
  • Frz
  • Fz
  • Fz1
  • frz
  • fz1
  • fz[[1]]
  • Fz-1
  • cFz-1
  • Xfrizzled-1
  • frizzled1
  • frz-1
  • frz1
  • fzd1-A
  • AW227548
  • FZ-1
  • frizzled
  • frizzled family receptor 1
  • frizzled homolog 1 (Drosophila)
  • fz
  • FZD1
  • Fzd1
  • fzd1
Epitop
N-Term
19
17
16
10
6
5
5
5
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Reaktivität
Fledermaus, Rind (Kuh), Hund, Hamster, Pferd, Human, Affe, Maus, Kaninchen, Ratte (Rattus)
92
67
3
2
2
2
2
Wirt
Kaninchen
65
52
3
Klonalität
Polyklonal
Konjugat
Dieser FZD1 Antikörper ist unkonjugiert
8
4
4
4
4
3
3
3
2
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
73
40
33
15
13
6
5
4
2
2
2
2
Hersteller
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Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN1048617
$ 451.00
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN2000335 IHC (p) ELISA Rabbit IgG AA 1-248, Extracellular Domain Anmelden zum Anzeigen Polyclonal

General

Antigen Frizzled Family Receptor 1 (Fzd1) Antikörper
Epitop N-Term
(19), (17), (16), (10), (6), (5), (5), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Fledermaus, Rind (Kuh), Hund, Hamster, Pferd, Human, Affe, Maus, Kaninchen, Ratte (Rattus)
(92), (67), (3), (2), (2), (2), (2)
Wirt Kaninchen
(65), (52), (3)
Klonalität
Polyklonal
Konjugat Dieser FZD1 Antikörper ist unkonjugiert
(8), (4), (4), (4), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(73), (40), (33), (15), (13), (6), (5), (4), (2), (2), (2), (2)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-FZD1 Antikörper

Target Details FZD1 Anwendungsinformationen Handhabung Bilder
Marke IHC-plus™
Spezifität Human FZD1 / Frizzled 1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Homologie Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Baboon, Monkey, Marmoset, Mouse, Rat, Shrew, Hamster, Panda, Dog, Bat, Bovine, Horse, Rabbit, Opossum, Armadillo (100%) Elephant (94%).
Reinigung Immunoaffinity purified
Immunogen Synthetic 17 amino acid peptide from N-terminal extracellular domain of human FZD1 / Frizzled 1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Baboon, Monkey, Marmoset, Mouse, Rat, Shrew, Hamster, Panda, Dog, Bat, Bovine, Horse, Rabbit, Opossum, Armadillo (100%), Elephant (94%).

Type of Immunogen: Synthetic peptide

Target Details FZD1

Produktdetails anti-FZD1 Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung FZD1 / Frizzled 1 (Fzd1 Antibody Abstract)
Hintergrund Name/Gene ID: FZD1
Subfamily: Frizzled
Family: GPCR

Synonyms: FZD1, Chfz-1, Fz1, Frizzled 1, Fz receptor 1, HFz1, Frizzled homolog 1, Fz-1, FzE1, Rfz1, Xfrizzled-1, Wnt receptor, Dfrizzled-1, Frizzled family receptor 1, Frizzled-1
Gen-ID 8321
Pathways WNT Signalweg, Asymmetric Protein Localization

Anwendungsinformationen

Produktdetails anti-FZD1 Antikörper Target Details FZD1 Handhabung Bilder zurück nach oben
Applikationshinweise Approved: IHC-P (7 μg/mL)
Kommentare

This antibody has been further validated specifically for use under standardized IHC conditions against formalin-fixed paraffin-embedded human tissues.

Testdurchführung The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
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Handhabung

Produktdetails anti-FZD1 Antikörper Target Details FZD1 Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze thaw cycles.
Haltbarkeit 12 months

Bilder

Produktdetails anti-FZD1 Antikörper Target Details FZD1 Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Frizzled Family Receptor 1 (Fzd1) (N-Term) antibody (ABIN1048617) Anti-FZD1 / Frizzled 1 antibody ABIN1048617 IHC staining of human prostate. Immunohi...