anti-Human EPH Receptor B2 Antikörper für Immunohistochemistry (Frozen Sections)

Recommended EPH Receptor B2 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
EPH Receptor B2 (EPHB2) Antikörper
  • CAPB
  • DRT
  • EK5
  • EPHT3
  • ERK
  • Hek5
  • PCBC
  • Tyro5
  • Cek5
  • Drt
  • ETECK
  • Erk
  • Nuk
  • Prkm5
  • Qek5
  • Sek3
  • RGD1564232
  • EPH receptor B2
  • Eph receptor B2
  • EPHB2
  • LOC100218346
  • Ephb2
Reaktivität
Human, Maus
163
58
5
3
3
3
3
3
2
2
1
1
1
1
1
Wirt
Maus
83
42
25
20
Klonalität
Monoklonal
Konjugat
Dieser EPH Receptor B2 Antikörper ist unkonjugiert
10
6
6
6
6
6
3
2
2
2
2
2
2
1
1
Applikation
Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
120
91
51
43
29
29
15
7
2
1
1
1
1
Optionen
Hersteller
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Produktnummer ABIN2689499
269,50 €
Zzgl. Versandkosten 20,00 € und MWSt

anti-EPH Receptor B2 Antikörper für Immunohistochemistry (Frozen Sections) mit den meisten Publikationen

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Applikation / Reaktivität Maus Human
ELISA 17 Antikörper 88 Antikörper
Enzyme Immunoassay (EIA) 1 Antikörper 7 Antikörper
Flow Cytometry (FACS) 27 Antikörper 40 Antikörper
Immunochromatography (IC) 1 Antikörper
Immunocytochemistry (ICC) 5 Antikörper 14 Antikörper
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antikörper
Immunohistochemistry (IHC) 10 Antikörper
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antikörper
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 8 Antikörper
Immunoprecipitation (IP) 1 Antikörper
Simple Western (SimWes) 1 Antikörper
Western Blotting (WB) 26 Antikörper

General

Antigen EPH Receptor B2 (EPHB2) Antikörper
Reaktivität Human, Maus
(163), (58), (5), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Wirt Maus
(83), (42), (25), (20)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser EPH Receptor B2 Antikörper ist unkonjugiert
(10), (6), (6), (6), (6), (6), (3), (2), (2), (2), (2), (2), (2), (1), (1)
Applikation Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(120), (91), (51), (43), (29), (29), (15), (7), (2), (1), (1), (1), (1)
Pubmed 4 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-EPH Receptor B2 Antikörper

Target Details EPH Receptor B2 Anwendungsinformationen Handhabung ProductDetails: References for anti-EPH Receptor B2 antibody (ABIN2689499) Bilder
Marke BD Pharmingen™
Produktmerkmale The 2H9 monoclonal antibody specifically binds to the Ephrin Type-B Receptor 2 (EphB2). EphB2 is a type I transmembrane glycoprotein that belongs to the Eph receptor family of tyrosine kinase receptors. EphB2 serves as a cell surface receptor tyrosine kinase for membrane-anchored ligands referred to as type B ephrins (ephrin-B). The EphB2 receptor can bind to ephrin-B1, ephrin-B2, and ephrin-B3. Transmembrane ephrin-B family members are key regulators of embryogenesis including development of the nervous and vascular systems. The EphB2 receptor functions as a chemodirectant in regulating cellular migration. EphB2/ephrin-B interactions orchestrate cell positioning by regulating cellular adhesion and repulsion during development, thereby influencing cell fate, morphogenesis and organogenesis. Signaling can occur in a forward pathway when the EphB2 receptor tyrosine kinase is activated by bound ligand and in a reverse pathway when transmembrane ephrin-B ligands are activated by EphB2 receptor-mediated crosslinking. In the adult body, Eph receptor signaling plays major roles in regulating the architecture and physiology of different tissues under normal as well as disease conditions such as cancer. Ephrin-B1 and ephrin-B2 levels are upregulated in the vasculature during inflammation. Ephrin-B2 Molecules that are localized to the luminal endothelial surface can signal through the EphB2 which is expressed by monocytes. This interaction promotes monocyte differentiation into proinflammatory macrophages. In the intestinal epithelium, EphB2/ephrin-B interactions regulate both cell positioning and tumor progression. The differential expression patterns of EphB2 allows for the detection and isolation of various intestinal epithelial cell types. These include intestinal stem cells (ISCs) which express high levels of EphB2. The 2H9 antibody reportedly blocks the interaction of EphB2 with ephrin ligands and crossreacts with mouse EphB2. Flow cytometric analysis of EphB2 expression on human Colo-205 cells. Cells from the human COLO 205 (Colorectal adenocarcinoma, ATCC CCL-222) cell line were stained with either Purified Mouse IgG1, κ Isotype Control (Cat. No. 550878, dashed line histogram) or Purified Mouse Anti-Human EphB2 antibody (Cat. No. 564698, solid line histogram). The cells were washed and counterstained with PE Goat Anti-Mouse Ig (Cat. No. 550589). The fluorescence histograms showing EphB2 expression (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System. Immunohistochemical analysis of human EphB2 expression in human placenta. Acetone-fixed frozen human placenta sections were stained with either Purified Mouse IgG1 κ Isotype Control (Cat No.550878, Left Image) or Purified Mouse Anti-Human EphB2 antibody (Right Image). A three-step staining procedure that employed Biotin Goat Anti-Mouse Immunoglobulin (Cat. No. 550337), Streptavidin-Horseradish Peroxidase (HRP) (Cat. No. 550946), and the DAB Substrate Kit (Cat. No. 550880) was used to develop the primary staining reagents. Original magnification:20x. 564698 Rev. 1 Page 1 of 2

BD Pharmingen™ Purified Mouse Anti-Human EphB2 - Purified - Clone 2H9 - Isotype Mouse IgG1, κ - Reactivity Hu, Ms - 0.1 mg
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human EphB2 Recombinant Protein
Klon 2H9
Isotyp IgG1, kappa

Target Details EPH Receptor B2

Produktdetails anti-EPH Receptor B2 Antikörper Anwendungsinformationen Handhabung ProductDetails: References for anti-EPH Receptor B2 antibody (ABIN2689499) Bilder zurück nach oben
Antigen
Andere Bezeichnung EphB2 (EPHB2 Antibody Abstract)
Hintergrund Synonyms: EPHB2, EphB2R, TYRO5, CAPB, DRT, EK5, EPHT3, EPTH3, HEK5, PCBC
Pathways RTK Signalweg, Regulation of long-term Neuronal Synaptic Plasticity

Anwendungsinformationen

Produktdetails anti-EPH Receptor B2 Antikörper Target Details EPH Receptor B2 Handhabung ProductDetails: References for anti-EPH Receptor B2 antibody (ABIN2689499) Bilder zurück nach oben
Applikationshinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-EPH Receptor B2 Antikörper Target Details EPH Receptor B2 Anwendungsinformationen ProductDetails: References for anti-EPH Receptor B2 antibody (ABIN2689499) Bilder zurück nach oben
Konzentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C
Informationen zur Lagerung Store undiluted at 4°C.

ProductDetails: References for anti-EPH Receptor B2 antibody (ABIN2689499)

Produktdetails anti-EPH Receptor B2 Antikörper Target Details EPH Receptor B2 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Jung, Sato, Merlos-Suárez, Barriga, Iglesias, Rossell, Auer, Gallardo, Blasco, Sancho, Clevers, Batlle: "Isolation and in vitro expansion of human colonic stem cells." in: Nature medicine, Vol. 17, Issue 10, pp. 1225-7, 2011

Merlos-Suárez, Barriga, Jung, Iglesias, Céspedes, Rossell, Sevillano, Hernando-Momblona, da Silva-Diz, Muñoz, Clevers, Sancho, Mangues, Batlle: "The intestinal stem cell signature identifies colorectal cancer stem cells and predicts disease relapse." in: Cell stem cell, Vol. 8, Issue 5, pp. 511-24, 2011

Foster, Gordon, Cardenas, Veiga-Fernandes, Makinen, Grigorieva, Wilkinson, Blackburn, Richie, Manley, Adams, Kioussis, Coles: "EphB-ephrin-B2 interactions are required for thymus migration during organogenesis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 30, pp. 13414-9, 2010

Mao, Luis, Ross, Silva, Tan, Crowley, Chui, Franz, Senter, Koeppen, Polakis: "EphB2 as a therapeutic antibody drug target for the treatment of colorectal cancer." in: Cancer research, Vol. 64, Issue 3, pp. 781-8, 2004