anti-Rind (Kuh) Profilin 1 Antikörper für Simple Western

Recommended Profilin 1 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Profilin 1 (PFN1) Antikörper
  • GmPRO1
  • GmPRO2
  • PRO1
  • Profilin-1
  • profilin-1
  • profilin
  • MGC130883
  • Profilin1
  • XProfilin1
  • sb:cb813
  • wu:fa91a03
  • ALS18
  • Pfn
  • F6F22.21
  • F6F22_21
  • PFN1
  • PROFILIN 1
  • profilin 1
  • profilin
  • profilin 1
  • profilin 1-like
  • profilin homolog1
  • profilin-1-like
  • Protein PFN-1
  • PRO2
  • PFN1
  • pfn1
  • Pfn1
  • PRF1
  • LOC100350198
  • prf1
  • LOC607397
  • pfn-1
Epitop
C-Term, AA 100-140
21
17
15
5
5
4
3
2
2
1
Reaktivität
Rind (Kuh), Human, Maus, Ratte (Rattus)
93
58
55
24
4
4
3
3
2
1
1
1
1
1
1
1
Wirt
Kaninchen
92
13
Klonalität
Polyklonal
Konjugat
Unkonjugiert
10
9
6
2
2
2
2
2
2
1
1
1
1
1
Applikation
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
87
46
31
26
12
12
12
4
4
3
2
1
Hersteller
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Bilder

Produktnummer ABIN446701
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General

Antigen Profilin 1 (PFN1) Antikörper
Epitop C-Term, AA 100-140
(21), (17), (15), (5), (5), (4), (3), (2), (2), (1)
Reaktivität Rind (Kuh), Human, Maus, Ratte (Rattus)
(93), (58), (55), (24), (4), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Wirt Kaninchen
(92), (13)
Klonalität
Polyklonal
Konjugat Unkonjugiert
(10), (9), (6), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Applikation Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
(87), (46), (31), (26), (12), (12), (12), (4), (4), (3), (2), (1)
Pubmed 1 Publikation vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails

Antigendetails Anwendungsinformationen Handhabung Referenzen Bilder
Reinigung Immunogen affinity purified
Immunogen Synthetic peptide made to a C-terminal portion of human Profilin 1 (within residues 100-140). [Swiss-Prot# P07737]

Antigendetails

Produktdetails Anwendungsinformationen Handhabung Referenzen Bilder zurück nach oben
Antigen
Andere Bezeichnung Profilin 1 (PFN1 Antibody Abstract)
Hintergrund Gene Symbol: PFN1
Molekulargewicht Theoretical MW: 14 kDa
Gen-ID 5216
UniProt P07737
Forschungsgebiet Neurology, Microfilaments, Cytoskeleton
Pathways Regulation of Actin Filament Polymerization, Tube Formation, Maintenance of Protein Location

Anwendungsinformationen

Produktdetails Antigendetails Handhabung Referenzen Bilder zurück nach oben
Applikationshinweise Western Blot 0.5 μg/mL, Simple Western 1:200, Immunohistochemistry 1:100, Immunohistochemistry-Paraffin 1:100This Profilin 1 antibody is useful for Immunohistochemistry on formalin fixed paraffin embedded sections and Western blot, where a band is seen at approx. 14 kDa. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Kommentare

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protokoll Western Blot protocol specific for Profilin 1 Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 % MES) on samples to be analyzed, loading 30 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % NFDM + 1 % BSA in TBS + Tween, 1 hour at RT.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the rabbit anti-Profilin primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).**Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.Immunohistochemistry-paraffin embedded sections protocol Immunohistochemistry-paraffin embedded sectionsAntigen UnmaskingBring slides to a boil in 10 mM sodium citrate buffer pH 6.0 then maintain at a sub-boiling temperature for 10 minutes.Cool slides on bench top for 30 minutes.Staining
. Wash sections in dH2O three times for 5 minutes each.
. Wash section in wash buffer (1X PBS/0.1 % Tween-20 (1X PBST)) for 5 minutes.
. Block each section with 100-400 µL blocking solution (1X PBST, 5 % goat serum) for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL primary antibody diluted in 1X PBST, 5 % goat serum to each section. Incubate overnight at 4C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated secondary antibody, diluted in 1X PBST, 5 % goat serum. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Striptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in dH2O.
. Counterstain sections in hematoxylin.
. Wash sections in dH2O two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails Antigendetails Anwendungsinformationen Referenzen Bilder zurück nach oben
Format Liquid
Konzentration 1.37 mg/mL
Buffer PBS and 30 % Glycerol
Buffer contains: 0.1 % Sodium Azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid freeze-thaw cycles
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Referenzen

Produktdetails Antigendetails Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

van Blitterswijk, Baker, Bieniek, Knopman, Josephs, Boeve, Caselli, Wszolek, Petersen, Graff-Radford, Boylan, Dickson, Rademakers: "Profilin-1 mutations are rare in patients with amyotrophic lateral sclerosis and frontotemporal dementia." in: Amyotrophic lateral sclerosis & frontotemporal degeneration, Vol. 14, Issue 5-6, pp. 463-9, 2013 (Probematerial (Species): Human). Weitere Details: Immunohistochemistry (Paraffin-embedded Sections)

Bilder

Produktdetails Antigendetails Anwendungsinformationen Handhabung Referenzen zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Profilin 1 (PFN1) (AA 100-140), (C-Term) antibody (ABIN446701) anti-Profilin 1 (PFN1) (AA 100-140), (C-Term) antibody
Western Blotting (WB) image for anti-Profilin 1 (PFN1) (AA 100-140), (C-Term) antibody (ABIN446701) anti-Profilin 1 (PFN1) (AA 100-140), (C-Term) antibody (Image 2)
Simple Western (SimWes) image for anti-Profilin 1 (PFN1) (AA 100-140), (C-Term) antibody (ABIN446701) Simple Western: Profilin 1 Antibody [NBP1-19344] - Simple Western lane view shows a s...