GDNF Protein (AA 78-211, N-Term)

Produktnummer ABIN2667420

Produktdetails

Target: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: N-Term, AA 78-211

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Flow Cytometry (FACS)

Reinheit: >98 % , as determined by Coomassie stained SDS-PAGE.

Endotoxin-Niveau:

Less than 0.1 ng per μg of protein.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Biological activity: ED50 ≤0.1 ng/ml, corresponding to a specific activity of ≥ 1.0 x 107 units/mg, as measured by its ability to stimulate proliferation of rat C6 cells in a dose dependent manner.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer, such as 5 % Trehalose, and store in working aliquots at -20 °C to -80 °C.

Buffer: Lyophilized, carrier-free.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Unopened vial can be stored at -20°C or -70°C.

Antigendetails

Target: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Andere Bezeichnung: GDNF (GDNF Produkte)

Synonyme: gdnf Protein, GDNF Protein, AI385739 Protein, gdnfa Protein, hfb1-gdnf Protein, ATF1 Protein, ATF2 Protein, HFB1-GDNF Protein, HSCR3 Protein, glial cell derived neurotrophic factor Protein, glial cell derived neurotrophic factor a Protein, glial cell line derived neurotrophic factor Protein, glial cell derived neurotrophic factor L homeolog Protein, Gdnf Protein, gdnfa Protein, GDNF Protein, gdnf.L Protein

Hintergrund: Glial cell line-derived neurotrophic factor (GDNF) is a member of the TGF-β superfamily and was first identified as a growth factor supporting the survival of embryonic midbrain neurons. GDNF has been shown to promote the development, differentiation, and survival of dopaminergic neurons by signaling through GDNF family receptor α-1 (GFRα1) and the coreceptor Ret receptor tyrosine kinase. Dysregulated expression of GDNF has been reported to be associated with several mental diseases, including depression and schizophrenia. GDNF is considered as a promising therapeutic agent for the treatment of Parkinson's disease, since GNDF can protect dopaminergic neurons from neurotoxin and promotes neuron regeneration after injury. In addition to the functions in the nervous system, GDNF is also required for the differentiation of spermatogonia and development of kidney. Upregulated expression of GNDF was observed in various cancer cell lines and correlates with malignant cancer progression, suggesting a role of GDNF in carcinogenesis.

Molekulargewicht: The 135 amino acid recombinant protein has a predicted molecular mass of approximately 15.0 kDa. Recombinant human GDNF forms a a disulfide-linked homodimer. The predicted N-terminal amino acid is Met.

Pathways: RTK Signalweg, Synaptic Membrane, Tube Formation, Autophagie, Smooth Muscle Cell Migration