anti-Maus RPS6KB1 Antikörper für Functional Studies

Recommended RPS6KB1 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) Antikörper
  • PS6K
  • S6K
  • S6K-beta-1
  • S6K1
  • STK14A
  • p70 S6KA
  • p70(S6K)-alpha
  • p70-S6K
  • p70-alpha
  • XPKAbeta
  • pkacb
  • 10539
  • 7084
  • CG10539
  • DS6K
  • Dmel\\CG10539
  • Dp70S6k
  • Dp70[s6k]
  • Dp70s6k
  • S6k
  • dS6K
  • dS6k
  • dp70[S6k]
  • dp70s6k
  • dps6k
  • ds6k
  • fs(3)07084
  • l(3)07084
  • p70 S6K
  • p70/S6K
  • p70S6K
  • p70[S6 kinase]
  • p70[S6K]
  • p70[S6k]
  • p70[S6kinase]
  • p70s6K
  • s6k
  • s6k11
  • 2610318I15Rik
  • 4732464A07Rik
  • 70kDa
  • AA959758
  • AI256796
  • AI314060
  • p70/85s6k
  • p70s6k
  • p70s6k-A
  • ribosomal protein S6 kinase B1
  • protein kinase cAMP-activated catalytic subunit beta
  • Ribosomal protein S6 kinase
  • ribosomal protein S6 kinase, polypeptide 1
  • ribosomal protein S6 kinase B1 L homeolog
  • RPS6KB1
  • prkacb
  • S6k
  • Rps6kb1
  • rps6kb1.L
Epitop
AA 26-43
108
39
37
30
28
21
21
20
20
18
18
17
15
15
15
14
14
14
12
10
10
9
8
6
6
6
6
5
5
5
5
5
4
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
701
437
414
73
54
41
34
33
21
13
13
8
5
5
3
3
1
Wirt
Kaninchen
628
53
23
2
Klonalität
Polyklonal
Konjugat
Dieser RPS6KB1 Antikörper ist unkonjugiert
24
22
21
14
13
13
9
9
9
9
9
8
8
8
8
8
8
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Functional Studies (Func), Immunoprecipitation (IP), Western Blotting (WB)
539
275
257
146
133
104
66
42
22
20
17
11
7
2
1
1
1
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
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Produktnummer ABIN211258
$ 947.83
Zzgl. Versandkosten $45.00

General

Antigen Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) Antikörper
Epitop AA 26-43
(108), (39), (37), (30), (28), (21), (21), (20), (20), (18), (18), (17), (15), (15), (15), (14), (14), (14), (12), (10), (10), (9), (8), (6), (6), (6), (6), (5), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human, Maus, Ratte (Rattus)
(701), (437), (414), (73), (54), (41), (34), (33), (21), (13), (13), (8), (5), (5), (3), (3), (1)
Wirt Kaninchen
(628), (53), (23), (2)
Klonalität
Polyklonal
Konjugat Dieser RPS6KB1 Antikörper ist unkonjugiert
(24), (22), (21), (14), (13), (13), (9), (9), (9), (9), (9), (8), (8), (8), (8), (8), (8), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Functional Studies (Func), Immunoprecipitation (IP), Western Blotting (WB)
(539), (275), (257), (146), (133), (104), (66), (42), (22), (20), (17), (11), (7), (2), (1), (1), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-RPS6KB1 Antikörper

Target Details RPS6KB1 Anwendungsinformationen Handhabung Bilder
Spezifität Recognizes p70 S6 kinase Mr ~64kD. In some cell lysates, an additional protein may be detected at a higher molecular weight. Cross-reacts with rat, human and mouse.
Reinigung Protein A purified
Immunogen Synthetic peptide corresponding to residues 26-43 of rat S6 Kinase. The immunizing sequence is identical in mouse and human.

Type of Immunogen: Synthetic peptide
Isotyp IgG

Target Details RPS6KB1

Produktdetails anti-RPS6KB1 Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung RPS6KB1 / P70S6K / S6K (RPS6KB1 Antibody Abstract)
Hintergrund Name/Gene ID: RPS6KB1
Subfamily: P70 RSK
Family: Protein Kinase

Synonyms: RPS6KB1, 70 kDa S6 kinase, p70 S6KA, p70-alpha, p70-S6K, p70 S6 kinase alpha, p70s6 kinase, S6K-beta-1, STK14A, p70 ribosomal S6 kinase, p70 S6 kinase, alpha 1, p70 S6 kinase, alpha 2, p70 S6K-alpha, p70-S6K 1, p70S6K, p70S6K1, PS6K, Ribosomal protein S6 kinase I, S6K, S6K1, p70 ribosomal S6 kinase alpha, p70(S6K)-alpha
Gen-ID 6198
UniProt P23443
Pathways PI3K-Akt Signalweg, RTK Signalweg, AMPK Signaling, Regulation of Cell Size, Skeletal Muscle Fiber Development, Feeding Behaviour, G-protein mediated Events, Smooth Muscle Cell Migration, Interaction of EGFR with phospholipase C-gamma

Anwendungsinformationen

Produktdetails anti-RPS6KB1 Antikörper Target Details RPS6KB1 Handhabung Bilder zurück nach oben
Applikationshinweise Approved: Func, IP, WB

Usage: Suitable for use in Western Blot, Immunoprecipitation. Western Blot Analysis: 0.5-2.0 μg/mL detects p70 S6 kinase in RIPA lysates from HEK293, PC-12, and A431 cells. HEK293 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-p70 S6 kinase (2 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4 μg immunoprecipitates p70 S6 kinase from 500 μg of HEK293 RIPA lysate. Immunoprecipitation Kinase Assay: 5 μg immunoprecipitates active p70 S6 kinase from IGF-1 stimulated (15nM) human HEK293 cell lysate containing 1 uM microcystin. The assay was performed using a S6 Kinase Assay Kit, which uses specific substrate peptide as a kinase substrate and contains a separate inhibitor cocktail which blocks the activity of other serine/threonine kinases such as protein kinase A, protein kinase C, and calmodulin dependent kinases.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-RPS6KB1 Antikörper Target Details RPS6KB1 Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration Lot specific
Buffer 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeat freeze-thaw cycles.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Long term: -20°C
Short term: +4°C. Avoid repeat freeze-thaw cycles.