anti-Maus p53 Antikörper für Immunoprecipitation

Recommended p53 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Tumor Protein P53 (TP53) Antikörper
  • tp53
  • p53
  • TP53
  • Tp53
  • bbl
  • bfy
  • bhy
  • p44
  • TpMs
  • BCC7
  • LFS1
  • P53
  • TRP53
  • Trp53
  • Xp53
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • wu:fb40d06
  • zgc:111919
  • cellular tumor antigen p53
  • tumor protein p53
  • tumor supressor p53
  • p53 tumor suppressor
  • transformation related protein 53
  • transformation related protein 53, pseudogene
  • trichoplein, keratin filament binding
  • Wistar clone pR53P1 p53 pseudogene
  • CpipJ_CPIJ002758
  • TP53
  • tp53
  • P53
  • Trp53
  • Trp53-ps
  • TCHP
  • Tp53
  • p53-ps
Epitop
full length
173
128
94
84
74
71
70
62
59
55
50
49
48
47
42
38
32
31
29
28
24
22
20
20
17
16
15
15
15
15
15
14
12
11
10
10
9
9
9
8
7
7
7
7
7
7
6
6
6
6
6
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
2428
518
425
188
127
94
87
84
35
23
20
18
17
16
15
14
6
4
4
4
4
3
3
3
2
2
1
1
Wirt
Maus
1372
1169
7
3
1
1
1
1
Klonalität
Monoklonal
Konjugat
Dieser p53 Antikörper ist unkonjugiert
81
78
65
55
49
48
34
34
26
26
25
25
25
25
25
24
24
24
23
22
22
21
14
14
14
14
14
12
7
5
5
5
5
5
5
5
5
4
3
2
2
2
Applikation
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Intracellular Staining (ICS), Immunoprecipitation (IP), Western Blotting (WB)
1931
772
751
742
651
637
434
347
315
179
72
41
32
31
24
17
17
15
13
9
8
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen

Bilder

Produktnummer ABIN967416
Preis und Verfügbarkeit auf Anfrage.
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN222890 ICC IP IHC WB Mouse IgG1 Ser20 Anmelden zum Anzeigen C6A5 2
1 ABIN4342783 FACS ICC IF IHC (fro) IHC (p) IP SimWes WB Mouse IgG2a AA 16-25, N-Term Anmelden zum Anzeigen BP53-12 1
1 ABIN152249 CyTOF ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Mouse IgG1 kappa AA 14-389 Anmelden zum Anzeigen PAb 240 20
1 ABIN4342746 IHC IP WB Rabbit pSer15 Anmelden zum Anzeigen Polyclonal 2
1 ABIN967419 IHC (fro) IHC (p) IP WB Mouse IgG1 AA 14-289 Anmelden zum Anzeigen PAb 240 7
1 ABIN967420 IHC (fro) IHC (p) IP WB Mouse IgG1 AA 14-289 Anmelden zum Anzeigen PAb 240 7
1 ABIN4342743 ICC IF IHC IHC (p) IP WB Rabbit pSer15 Anmelden zum Anzeigen Polyclonal 2
1 ABIN967409 FACS IF IP WB Mouse IgG2b Anmelden zum Anzeigen PAb 122 5
1 ABIN264401 EIA IP WB Mouse IgG1 AA 16-25 Anmelden zum Anzeigen X77 2
1 ABIN126922 IF IHC (p) IP WB Mouse IgG1 Ser20 Anmelden zum Anzeigen C6A5
1 ABIN264449 EIA IHC (fro) IP WB Mouse IgG2b AA 371-380 Anmelden zum Anzeigen HR231
1 ABIN223224 IP IHC WB Rabbit IgG pSer15 Anmelden zum Anzeigen Polyclonal
1 ABIN1078634 ICC IHC IP WB Rabbit IgG AA 137-390 Anmelden zum Anzeigen Polyclonal
1 ABIN4342781 FACS ICC IF IHC (fro) IHC (p) IP SimWes WB Mouse IgG2a AA 16-25, N-Term Anmelden zum Anzeigen BP53-12
1 ABIN4342782 FACS ICC IF IHC (fro) IHC (p) IP SimWes WB Mouse IgG2a AA 16-25, N-Term Anmelden zum Anzeigen BP53-12
1 ABIN127171 IHC (p) IP WB Rabbit pSer15 Anmelden zum Anzeigen Polyclonal
1 ABIN385160 FACS GS IF IHC (p) IP WB Mouse IgG2a AA 11-25 Anmelden zum Anzeigen DO-1
1 ABIN5563643 IHC IP WB Rabbit IgG pSer15 Anmelden zum Anzeigen Polyclonal
1 ABIN4342797 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 405 Mouse IgG2a AA 16-25, N-Term Anmelden zum Anzeigen BP53-12
1 ABIN4342799 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 647 Mouse IgG2a AA 16-25, N-Term Anmelden zum Anzeigen BP53-12

General

Antigen Tumor Protein P53 (TP53) Antikörper
Epitop full length
(173), (128), (94), (84), (74), (71), (70), (62), (59), (55), (50), (49), (48), (47), (42), (38), (32), (31), (29), (28), (24), (22), (20), (20), (17), (16), (15), (15), (15), (15), (15), (14), (12), (11), (10), (10), (9), (9), (9), (8), (7), (7), (7), (7), (7), (7), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human, Maus, Ratte (Rattus)
(2428), (518), (425), (188), (127), (94), (87), (84), (35), (23), (20), (18), (17), (16), (15), (14), (6), (4), (4), (4), (4), (3), (3), (3), (2), (2), (1), (1)
Wirt Maus
(1372), (1169), (7), (3), (1), (1), (1), (1)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser p53 Antikörper ist unkonjugiert
(81), (78), (65), (55), (49), (48), (34), (34), (26), (26), (25), (25), (25), (25), (25), (24), (24), (24), (23), (22), (22), (21), (14), (14), (14), (14), (14), (12), (7), (5), (5), (5), (5), (5), (5), (5), (5), (4), (3), (2), (2), (2)
Applikation Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Intracellular Staining (ICS), Immunoprecipitation (IP), Western Blotting (WB)
(1931), (772), (751), (742), (651), (637), (434), (347), (315), (179), (72), (41), (32), (31), (24), (17), (17), (15), (13), (9), (8), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 11 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-p53 Antikörper

Target Details p53 Anwendungsinformationen Handhabung Referenzen für anti-p53 Antikörper (ABIN967416) Bilder
Marke BD Pharmingen™
Kreuzreaktivität Maus, Ratte (Rattus)
Produktmerkmale 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Recombinant full-length human p53
Klon G59-12
Isotyp IgG1

Target Details p53

Produktdetails anti-p53 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-p53 Antikörper (ABIN967416) Bilder zurück nach oben
Antigen
Andere Bezeichnung p53 (TP53 Antibody Abstract)
Hintergrund P53 is a 53 kD nuclear phosphoprotein that acts as a tumor suppressor protein, and is involved in inhibiting cell proliferation when DNA damage occurs. The gene for p53 is the most commonly mutated gene yet identified in human cancers. Missense mutations occur in tumors of the colon, lung, breast, ovary, bladder and several other organs. The mutant p53 is overexpressed in a variety of transformed cells and the wildtype p53 forms specific complexes with several viral oncogenes including SV40 large T, E1B from adenovirus and E6 from human papilloma virus. Wildtype p53 plays a role as a checkpoint protein for DNA damage during the S-phase of the cell cycle. p53 migrates at a reduced molecular weight of 53 kDa. Clone G59-12 recognizes mutant and wild type human, rat and mouse p53 tumor suppressor protein. Recombinant full-length human p53 was used as immunogen.
Molekulargewicht 53 kDa
Forschungsgebiet Cancer, Cell Cycle, Transcription Factors, DNA/RNA, Apoptosis/Necrosis
Pathways p53 Signalweg, MAPK Signalweg, PI3K-Akt Signalweg, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagie

Anwendungsinformationen

Produktdetails anti-p53 Antikörper Target Details p53 Handhabung Referenzen für anti-p53 Antikörper (ABIN967416) Bilder zurück nach oben
Applikationshinweise Clone G59-12 conjugated to R-Phycoerythrin (PE) is suggested for flow cytometric analysis of p53. Positive control cell lines include SKBR-3 human breast carcinoma cells (ATCC HTB-30) and A431 human vulval carcinoma cells (ATCC CRL-1555). Jurkat T cells (ATCC TIB-152) or MCF-7 human breast carcinoma cells (ATCC HTB-22) are suggested as negative controls. Positive immunostaining is seen in a high proportion of breast and colon carcinomas. p53 staining is not typically detected in normal skin, brain, kidney, lung, stomach, or breast tissue.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-p53 Antikörper Target Details p53 Anwendungsinformationen Referenzen für anti-p53 Antikörper (ABIN967416) Bilder zurück nach oben
Format Liquid
Konzentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C
Informationen zur Lagerung Store undiluted at 4°C.

Referenzen für anti-p53 Antikörper (ABIN967416)

Produktdetails anti-p53 Antikörper Target Details p53 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Van Meir, Roemer, Diserens, Kikuchi, Rempel, Haas, Huang, Friedmann, de Tribolet, Cavenee: "Single cell monitoring of growth arrest and morphological changes induced by transfer of wild-type p53 alleles to glioblastoma cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 4, pp. 1008-12, 1995

Jacquemier, Molès, Penault-Llorca, Adélaide, Torrente, Viens, Birnbaum, Theillet: "p53 immunohistochemical analysis in breast cancer with four monoclonal antibodies: comparison of staining and PCR-SSCP results." in: British journal of cancer, Vol. 69, Issue 5, pp. 846-52, 1994

Mørkve, Halvorsen, Stangeland, Gulsvik, Laerum: "Quantitation of biological tumor markers (p53, c-myc, Ki-67 and DNA ploidy) by multiparameter flow cytometry in non-small-cell lung cancer." in: International journal of cancer. Journal international du cancer, Vol. 52, Issue 6, pp. 851-5, 1993

van den Berg, Baas, Polak, Offerhaus: "Detection of p53 overexpression in routinely paraffin-embedded tissue of human carcinomas using a novel target unmasking fluid." in: The American journal of pathology, Vol. 142, Issue 2, pp. 381-5, 1993

Yeargin, Cheng, Yu, Gjerset, Bogart, Haas: "P53 mutation in acute T cell lymphoblastic leukemia is of somatic origin and is stable during establishment of T cell acute lymphoblastic leukemia cell lines." in: The Journal of clinical investigation, Vol. 91, Issue 5, pp. 2111-7, 1993

Cheng, Yee, Yeargin, Friedmann, Haas: "Suppression of acute lymphoblastic leukemia by the human wild-type p53 gene." in: Cancer research, Vol. 52, Issue 1, pp. 222-6, 1992

Yeargin, Cheng, Haas: "Role of the p53 tumor suppressor gene in the pathogenesis and in the suppression of acute lymphoblastic T-cell leukemia." in: Leukemia, Vol. 6 Suppl 3, pp. 85S-91S, 1992

Vogelstein: "Cancer. A deadly inheritance." in: Nature, Vol. 348, Issue 6303, pp. 681-2, 1991

Allgemeine Veröffentlichungen

Vojt?sek, Bártek, Midgley, Lane: "An immunochemical analysis of the human nuclear phosphoprotein p53. New monoclonal antibodies and epitope mapping using recombinant p53." in: Journal of immunological methods, Vol. 151, Issue 1-2, pp. 237-44, 1992

Gjerset, Arya, Volkman, Haas: "Association of induction of a fully tumorigenic phenotype in murine radiation-induced T-lymphoma cells with loss of differentiation antigens, gain of CD44, and alterations in p53 protein levels." in: Molecular carcinogenesis, Vol. 5, Issue 3, pp. 190-8, 1992

Stein, Stoica, Tilley, Burghardt: "Rat ovarian granulosa cell culture: a model system for the study of cell-cell communication during multistep transformation." in: Cancer research, Vol. 51, Issue 2, pp. 696-706, 1991

Bilder

Produktdetails anti-p53 Antikörper Target Details p53 Anwendungsinformationen Handhabung Referenzen für anti-p53 Antikörper (ABIN967416) zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-Tumor Protein P53 (TP53) (full length) antibody (ABIN967416) Western blot analysis of p53. A SV-40 transformed rat granulosa cell lysate was probe...
 image for anti-Tumor Protein P53 (TP53) (full length) antibody (ABIN967416) anti-Tumor Protein P53 (TP53) (full length) antibody (Image 2)
'Independent Validation' Siegel
Chargennummer 2335876
Validierte Anwendung Western Blotting
Positivkontrolle U251-MG cells
Negativkontrolle PC3 cells
Bewertung Strong bands of the expected size were observed in the positive control sample, and not in the negative control sample.
Primärantikörper
Sekundärantikörper
Kontrollen
  • U251-MG (positive) and PC3 (negative) cell line extracts were prepared using RIPA buffer (R0278, Sigma Aldrich).
  • Loading control: blots were stripped and re-probed for beta-actin to ensure equal loading of lysates.
Protokoll
  • 1. Total protein extracts were boiled in 1X SDS Sample Buffer containing 1% SDS and 1.25%
  • Beta-mercaptoethanol at 95°C for 5 min prior to loading.
  • 2. 32 µg of boiled extracts were loaded and resolved on a 8-16% SDS-polyacrylamide gel.
  • 3. The Spectra Multicolor Broad Range molecular mass marker (26634 Thermo Scientific) was used as a
  • standard.
  • 4. Proteins were then transferred onto PVDF membrane by tank transfer and protein transfer was
  • confirmed with Ponceau S staining.
  • 5. The immunoblot membrane was blocked in PBS containing 3% (W/V) non-fat dry milk at room
  • temperature for 1 h.
  • 6. The membrane was rinsed with PBS containing 0.05% Tween-20 once.
  • 7. The membrane was immersed with the protein side up in the antibody solution in PBS containing 1%
  • (W/V) non-fat dry milk and incubated for 2 h at room temperature (~26°C).
  • 8. The membrane was rinsed in PBS containing 0.05% Tween-20 thrice for 10 min each.
  • 9. The membrane was incubated in the HRP-conjugated secondary antibody solution in PBS containing
  • 1% (W/V) non-fat dry milk and incubated for 1 h at room temperature (~26°C) with gentle agitation.
  • 10. The membrane was rinsed thrice PBS containing 0.05% Tween-20 thrice for 10 min each.
  • 11. The membrane was washed in PBS twice for 30 sec each.
  • 12. Signals were detected with Pierce ECL Western Blotting Substrate (32109, Thermo Scientific). The
  • blot was scanned for 300 sec.
  • 13. The membrane was rinsed three times with PBS containing 0.05% Tween-20.4. Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10
  • min each.
  • 15. The membrane was washed in PBS containing 0.05% Tween-20 times for 10 min each.
  • 16. Repeated Steps 5-12 with the loading control antibody (beta-actin) and its matching secondary
  • antibody.
Anmerkungen None
Validierungsbilder
Western Blotting validation image for anti-Tumor Protein P53 (TP53) (full length) antibody (ABIN967416) Figure 1: Western blot analysis of U251-MG and PC3 cell line extracts using Tumor Pro...