Interleukin 6 (IL6) ELISA Kit

Antigen: Interleukin 6 (IL6)

Synonyme: HGF, HSF, BSF2, IL-6, IFNB2, Il-6, ILg6, Ifnb2, IL6, il-6

Reaktivität: Human

Applikation: ELISA

Zertifikate: ISO 13485:2003

Produktnummer: ABIN577085

Produktbeschreibung

Weitere Bezeichnung: Interleukin-6 (IL-6)

Beschreibung: Hepatitis B is an infection of the liver caused by the hepatitis B virus. Approximately 5-1% of adults and 9% of babies who are infected with HBV will go on to carry the virus for the rest of their lives. These people will pass the virus onto others. HBV is excreted in body fluids such as semen, saliva, blood and urine in persons with acute or chronic infection. The route of transmission can include homosexual or heterosexual activity, blood-borne exposure (needles, transfusion), mother-infant, close personal contact and even by consuming contaminated food or water. Thus, Hepatitis B has become a major public health concern. When HBV invades the body it causes liver damage through induction of auto-immunity. Liver cell injury results from cytotoxic T cell activity rather than viral cytotoxic activity. The principle screening test for detecting current (acute or chronic) HBV infection is the identification of HBsAg, an envelope lipoprotein. This is the first immunological marker, to appear in a patient's serum and exist in high quantities in the blood. Patients who are HBsAg positive develop chronic persistent hepatitis (CPH) and chronic active hepatitis (CAH). Patients with CPH usually remain in good health but those with CAH have progressive liver damage with the outcome being portal fibrosis, cirrhosis, or hepatocellular carcinoma. Screening for HBsAg is recommended for all donors, pregnant women and those individuals who at high risk. Presence of this marker is conclusive proof of HBV infection. YBL offers an immunoassay that can measure HBsAg through antigen-antibody interactions

Sensitivität: The minimum detectable dose of IL-6 was determined by adding two standard deviations to the mean optical density value of the zero standard replicates and calculating the corresponding concentration from the standard curve. The minimum detectable dose using a standard curve generated with Calibrator Diluent I is 2.0 pg/mL and using Calibrator Diluent II is 1.75 pg/mL.

Anwendungen

Prinzip: This IL-6 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to IL-6. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-6 and incubated. IL-6 if present, will bind and become immobilized by the antibody pre-coated on the wells and then be "sandwiched" by biotin conjugate. The microtiter plate wells are thoroughly washed to remove unbound IL-6 and other components of the sample. In order to quantitatively determine the amount of IL-6 present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is a tetramer containing four identical subunits that each have a high affinity-binding site for biotin. The wells are thoroughly washed to remove all unbound HRP-conjugated Avidin and a TMB (3,3'5, 5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450nm 2 nm. In order to measure the concentration of IL-6 in the samples this kit includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing.) According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus IL-6 concentration (pg/mL). The concentration of IL-6 in the samples is then determined by comparing the O.D. of the samples to the standard curve. S7.5(03) IL-6 3 LIMITATIONS OF THE PROCEDURE FOR LABORATORY RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES. As manufacturers we take great care to ensure that our products are suitable for use with all validated sample types, as designated in the product insert. However, it is possible that in some cases, high levels of interfering factors may cause unusual results. The kit should not be used beyond the expiration date on the kit label. It is important that the Calibrator Diluent selected for the standard curve be consistent with the samples being assayed. If samples generate values higher than the highest standard, dilute the samples with the appropriate Calibrator Diluent and repeat the assay. Any variation in standard diluent, operator, pipetting technique, washing technique, incubation time or temperature, and kit age can cause variation in binding. Soluble receptors or other binding proteins present in biological samples do not necessarily interfere with the measurement of ligands in samples. However, until the factors have been tested, the possibility of interference cannot be excluded

Beschränkungen: Nur für Forschungszwecke einsetzbar