anti-Maus Phosphoglycerate Kinase 1 Antikörper für Enzyme Immunoassay

Recommended Phosphoglycerate Kinase 1 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Phosphoglycerate Kinase 1 (PGK1) Antikörper
  • PGK1
  • PGK
  • MIG10
  • PGKA
  • Pgk-1
  • Pgk
  • wu:fd59b07
  • wu:fj36g06
  • zgc:56252
  • zgc:77899
  • phosphoglycerate kinase 1
  • phosphoglycerate kinase 1
  • PGK1
  • LOC100348124
  • Pgk1
  • pgk1
Epitop
Middle Region
21
13
11
6
6
6
5
3
3
3
2
2
2
2
1
1
1
1
1
1
1
Reaktivität
Human, Maus
135
67
41
5
5
4
4
4
4
4
3
3
2
2
1
1
1
1
Wirt
Kaninchen
117
17
4
1
Klonalität
Polyklonal
Konjugat
Unkonjugiert
8
7
7
4
4
4
1
1
1
1
1
1
1
1
1
1
1
Applikation
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
115
69
53
27
21
18
13
11
6
4
3
3
3
2
1
1
Hersteller
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Bilder

Produktnummer ABIN359199
$ 390.50
Zzgl. Versandkosten $45.00

General

Antigen Phosphoglycerate Kinase 1 (PGK1) Antikörper
Epitop Middle Region
(21), (13), (11), (6), (6), (6), (5), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human, Maus
(135), (67), (41), (5), (5), (4), (4), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1)
Wirt Kaninchen
(117), (17), (4), (1)
Klonalität
Polyklonal
Konjugat Unkonjugiert
(8), (7), (7), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(115), (69), (53), (27), (21), (18), (13), (11), (6), (4), (3), (3), (3), (2), (1), (1)
Pubmed 1 Publikation vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails

Antigendetails Anwendungsinformationen Handhabung Referenzen Bilder
Spezifität This antibody reacts to PGK1.
Reinigung Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human PGK1.
Isotyp Ig

Antigendetails

Produktdetails Anwendungsinformationen Handhabung Referenzen Bilder zurück nach oben
Antigen
Hintergrund Also known as ATP:3-phosphoglycerate 1-phosphotransferase (EC 2.7.2.3), this major enzyme in glycolysis catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate, generating one molecule of ATP. New blood vessel formation or angiogenesis is critical for tumor expansion and metastasis. Lay et al. (2000) showed that the plasmin reductase isolated from conditioned medium of fibrosarcoma cells is the glycolytic enzyme phosphoglycerate kinase. They concluded that phosphoglycerate kinase not only functions in glycolysis but is secreted by tumor cells and participates in the angiogenic process as a disulfide reductase.Synonyms: Cell migration-inducing gene 10 protein, PGKA, PRP 2, Primer recognition protein 2
Gen-ID 5230, 9606
UniProt P00558
Pathways Cellular Glucan Metabolic Process

Anwendungsinformationen

Produktdetails Antigendetails Handhabung Referenzen Bilder zurück nach oben
Applikationshinweise ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry 1/50 - 1/100. Flow cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails Antigendetails Anwendungsinformationen Referenzen Bilder zurück nach oben
Format Liquid
Konzentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Referenzen

Produktdetails Antigendetails Anwendungsinformationen Handhabung Bilder zurück nach oben
Allgemeine Veröffentlichungen

West, Moore, Biskup, Bugayenko, Smith, Ross, Dawson, Dawson: "Parkinson's disease-associated mutations in leucine-rich repeat kinase 2 augment kinase activity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 46, pp. 16842-7, 2005

Bilder

Produktdetails Antigendetails Anwendungsinformationen Handhabung Referenzen zurück nach oben
Bilder des Herstellers
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Phosphoglycerate Kinase 1 (PGK1) (Middle Region) antibody (ABIN359199) Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PGK1 a...
Western Blotting (WB) image for anti-Phosphoglycerate Kinase 1 (PGK1) (Middle Region) antibody (ABIN359199) The anti-PGK1 Pab is used in Western blot to detect PGK1 in mouse stomach tissue lysa...