anti-Maus Occludin Antikörper für Immunofluorescence (Paraffin-embedded Sections)

Recommended Occludin Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Occludin (OCLN) Antikörper
  • tpmt
  • OCLN
  • BLCPMG
  • AI503564
  • Ocl
  • ocln
  • oclnb
  • wu:fd23h10
  • wu:fi13c01
  • zgc:113992
  • zgc:56359
  • occludin
  • occludin a
  • OCLN
  • ocln
  • Ocln
  • ocln-b
  • oclna
Epitop
AA 480-520
34
14
13
7
7
6
5
5
4
4
3
3
2
2
1
1
Reaktivität
Human, Maus, Schwein, Ratte (Rattus)
111
55
49
17
16
4
4
4
3
2
2
2
Wirt
Kaninchen
85
30
3
Klonalität
Polyklonal
Konjugat
Dieser Occludin Antikörper ist unkonjugiert
6
6
5
3
3
3
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
100
55
29
25
21
12
10
7
3
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN687337
315,94 €
Zzgl. Versandkosten 20,00 € und MWSt
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN909382 IF (p) Alexa Fluor 350 Rabbit IgG AA 480-520 Anmelden zum Anzeigen Polyclonal
1 ABIN909384 IF (p) Alexa Fluor 488 Rabbit IgG AA 480-520 Anmelden zum Anzeigen Polyclonal
1 ABIN909383 IF (p) Alexa Fluor 555 Rabbit IgG AA 480-520 Anmelden zum Anzeigen Polyclonal
1 ABIN2802811 IF (p) Alexa Fluor 594 Rabbit IgG AA 480-520 Anmelden zum Anzeigen Polyclonal
1 ABIN909381 IF (p) Alexa Fluor 647 Rabbit IgG AA 480-520 Anmelden zum Anzeigen Polyclonal
1 ABIN687340 IF (p) Cy3 Rabbit IgG AA 480-520 Anmelden zum Anzeigen Polyclonal
1 ABIN687341 IF (p) Cy5 Rabbit IgG AA 480-520 Anmelden zum Anzeigen Polyclonal
1 ABIN687342 IF (p) Cy5.5 Rabbit IgG AA 480-520 Anmelden zum Anzeigen Polyclonal
1 ABIN687343 IF (p) Cy7 Rabbit IgG AA 480-520 Anmelden zum Anzeigen Polyclonal
1 ABIN687344 IF (p) FITC Rabbit IgG AA 480-520 Anmelden zum Anzeigen Polyclonal
1 ABIN5007977 IF (p) Alexa Fluor 680 Rabbit IgG AA 480-520 Anmelden zum Anzeigen Polyclonal
1 ABIN5007978 IF (p) Alexa Fluor 750 Rabbit IgG AA 480-520 Anmelden zum Anzeigen Polyclonal

anti-Occludin Antikörper für Immunofluorescence (Paraffin-embedded Sections) mit den meisten Publikationen

Ähnliche anti-Occludin Antikörper

Applikation / Reaktivität Maus Ratte (Rattus) Human Schwein
Immunofluorescence (fixed cells) (IF/ICC) 1 Antikörper
Western Blotting (WB) 36 Antikörper 94 Antikörper 42 Antikörper 5 Antikörper
Proximity Ligation Assay (PLA) 1 Antikörper
Immunoprecipitation (IP) 6 Antikörper 2 Antikörper 1 Antikörper
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 22 Antikörper 10 Antikörper 9 Antikörper 4 Antikörper
Immunohistochemistry (IHC) 11 Antikörper 21 Antikörper 13 Antikörper
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antikörper 1 Antikörper 1 Antikörper

General

Antigen Occludin (OCLN) Antikörper
Epitop AA 480-520
(34), (14), (13), (7), (7), (6), (5), (5), (4), (4), (3), (3), (2), (2), (1), (1)
Reaktivität Human, Maus, Schwein, Ratte (Rattus)
(111), (55), (49), (17), (16), (4), (4), (4), (3), (2), (2), (2)
Wirt Kaninchen
(85), (30), (3)
Klonalität
Polyklonal
Konjugat Dieser Occludin Antikörper ist unkonjugiert
(6), (6), (5), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(100), (55), (29), (25), (21), (12), (10), (7), (3), (1), (1), (1)
Pubmed 5 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-Occludin Antikörper

Target Details Occludin Anwendungsinformationen Handhabung ProductDetails: References for anti-Occludin antibody (ABIN687337) Bilder
Reinigung Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from mouse Occludin
Isotyp IgG

Target Details Occludin

Produktdetails anti-Occludin Antikörper Anwendungsinformationen Handhabung ProductDetails: References for anti-Occludin antibody (ABIN687337) Bilder zurück nach oben
Antigen
Andere Bezeichnung Occludin (OCLN Antibody Abstract)
Hintergrund

May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier.

Subcellular location: Cytoplasm

Synonyms: Ocl, AI53564, Occludin, Ocln

Gen-ID 18260
UniProt Q61146
Pathways Cell-Cell Junction Organization, Hepatitis C

Anwendungsinformationen

Produktdetails anti-Occludin Antikörper Target Details Occludin Handhabung ProductDetails: References for anti-Occludin antibody (ABIN687337) Bilder zurück nach oben
Applikationshinweise WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-Occludin Antikörper Target Details Occludin Anwendungsinformationen ProductDetails: References for anti-Occludin antibody (ABIN687337) Bilder zurück nach oben
Format Liquid
Konzentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20°C
Haltbarkeit 12 months

ProductDetails: References for anti-Occludin antibody (ABIN687337)

Produktdetails anti-Occludin Antikörper Target Details Occludin Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Sun, Liu, Xue, Wang: "Effects of insulin combined with idebenone on blood-brain barrier permeability in diabetic rats." in: Journal of neuroscience research, Vol. 93, Issue 4, pp. 666-77, 2015

Zhao, Qin, Han, Wang, Zhang, Liu: "?-Conglycinin reduces the tight junction occludin and ZO-1 expression in IPEC-J2." in: International journal of molecular sciences, Vol. 15, Issue 2, pp. 1915-26, 2014

Pan, Qin, Zhao, Wang, Liu, Che: "Effects of soybean agglutinin on mechanical barrier function and tight junction protein expression in intestinal epithelial cells from piglets." in: International journal of molecular sciences, Vol. 14, Issue 11, pp. 21689-704, 2013

Zhao, Qin, Sun, Che, Bao, Zhang: "Effects of soybean agglutinin on intestinal barrier permeability and tight junction protein expression in weaned piglets." in: International journal of molecular sciences, Vol. 12, Issue 12, pp. 8502-12, 2012

Gu, Xue, Wei, Zhang, Li: "Calcium-activated potassium channel activator down-regulated the expression of tight junction protein in brain tumor model in rats." in: Neuroscience letters, Vol. 493, Issue 3, pp. 140-4, 2011

Bilder

Produktdetails anti-Occludin Antikörper Target Details Occludin Anwendungsinformationen Handhabung ProductDetails: References for anti-Occludin antibody (ABIN687337) zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Occludin Antikörper (OCLN) (AA 480-520) (ABIN687337) Formalin-fixed and paraffin embedded mouse embryo tissue labeled with Anti-Occludin P...
Immunohistochemistry (IHC) image for anti-Occludin Antikörper (OCLN) (AA 480-520) (ABIN687337) Formalin-fixed and mouse kidney tissue labeled with Anti-Occludin Polyclonal Antibody...
Immunofluorescence (IF) image for anti-Occludin Antikörper (OCLN) (AA 480-520) (ABIN687337) Independently Validated Antibody, image provided by Science Direct, badge number 0296...
Western Blotting (WB) image for anti-Occludin Antikörper (OCLN) (AA 480-520) (ABIN687337) Human A549 lysates probed with Rabbit Anti-Occludin Polyclonal Antibody, Unconjugated...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Occludin Antikörper (OCLN) (AA 480-520) (ABIN687337) Formalin-fixed and paraffin embedded mouse embryo tissue labeled with Anti-Occludin P...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Occludin Antikörper (OCLN) (AA 480-520) (ABIN687337) Formalin-fixed and mouse kidney tissue labeled with Anti-Occludin Polyclonal Antibody...
'Independent Validation' Siegel
Chargennummer 990698W
Validierte Anwendung Immunofluorescence
Positivkontrolle Mouse stomach tissue
Negativkontrolle Human fat tissue
Bewertung Signal was detected in positive control sample and not in negative control sample.
Primärantikörper
Isotypkontrolle
Sekundärantikörper
Kontrollen
  • Positive control: PPFE mouse stomach tissue
  • Negative Control: PPFE human fat tissue
  • Isotype antibody control: tissue sections treated with Rabbit IgG Isotype at 1ug/ul , Bioss bs-0295p
  • Secondary antibody only control: positive and negative control tissue sections treated with Goat anti-Rabbit Alexa 647 secondary antibody only.
Protokoll
  • Paraffin embedded positive and negative control tissue sections were deparaffinized and undergone antigen retrieval using 10 mM Sodium Citrate pH6 in a pressure cooker for 10 min.
  • The tissue sections were incubated with 1 mg/mL Sodium borohydride for 10 min at room temperature to block autofluorescent background signal. The sections were then rinsed three times in TBS for 5 min each at RT.
  • Tissue sections were blocked in 1 X TBS / 5% normal donkey serum for 60 min at RT.
  • Tissue sections were incubated with primary antibody diluted 1:200 in 1X PBS / 5% normal donkey serum overnight at 4°C.
  • Tissue sections were rinsed three times in TBS for 5 min each at RT.
  • Cells were incubated with secondary antibody diluted 1:200 in 1X PBS / 5% normal donkey serum for 60 min at RT in dark.
  • Tissue sections were rinsed three times in TBS for 5 min each at RT.
  • Coverslips were mounted on slides with Prolong Gold anti-fade mounting media (Invitrogen)
  • IF stained tissue sections were imaged with a Zeiss LSM 510 Meta confocal microscope.
Anmerkungen
  • No challenges notes
  • OCLN is recommended to staining of mouse stomach tissue according to this protocol.
Validierungsbilder
Immunofluorescence validation image for anti-Occludin (OCLN) (AA 480-520) antibody (ABIN687337) Figure 1: Immunostaining (green) of mouse stomach tissue (A-C) and human fat tissue (...