Maus TNFSF12 ELISA Kit

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Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12) ELISA Kits
  • APO3L
  • DR3LG
  • TWEAK
  • Apo3l
  • Dr3l
  • Dr3lg
  • Tweak
  • tumor necrosis factor (ligand) superfamily, member 12
  • tumor necrosis factor ligand superfamily member 12
  • TNFSF12
  • Tnfsf12
Epitop
AA 94-249
Reaktivität
Maus
Alternativen
Kits mit alternativen Reaktivitäten:
15
8
8
Methodentyp
Sandwich ELISA
Detektionsbereich
15.6-1000 pg/mL
Untere Nachweisgrenze
15.6 pg/mL
Applikation
ELISA
Optionen
Hersteller
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Bilder

Produktnummer ABIN1889351
475,62 €
Zzgl. Versandkosten 20,00 € und MWSt
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Hersteller References Details
9.70292 ABIN625257 Sandwich ELISA Plasma, Cell Culture Supernatant, Serum 0.3-200 pg/mL 0.3 pg/mL Anmelden zum Anzeigen
9.70292 ABIN825006 Sandwich ELISA Serum, Plasma, Tissue Homogenate 15.6-1000 pg/mL 15.6 pg/mL Anmelden zum Anzeigen
9.70292 ABIN2708613 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 15.6-1000 pg/mL 15.6 pg/mL Anmelden zum Anzeigen
1 ABIN3032848 Competition ELISA Cell Culture Supernatant, Plasma, Serum 0.27-200 pg/mL 0.27 pg/mL Anmelden zum Anzeigen
1 ABIN5524729 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 15.625-1000 pg/mL 15.625 pg/mL Anmelden zum Anzeigen
1 ABIN2685743 Sandwich ELISA Plasma (heparin), Plasma (EDTA), Cell Culture Supernatant, Serum 15.6-1000 pg/mL 15.6 pg/mL Anmelden zum Anzeigen
1 ABIN2685744 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum Anmelden zum Anzeigen
1 ABIN1164362 Anmelden zum Anzeigen

TNFSF12 ELISA Kit für ELISA mit den meisten Publikationen

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Applikation / Reaktivität Human Ratte (Rattus)
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General

Antigen Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12) ELISA Kits
Epitop AA 94-249
Reaktivität Maus
Kits mit alternativen Reaktivitäten:
(15), (8), (8)
Methodentyp Sandwich ELISA
Detektionsbereich 15.6-1000 pg/mL
Untere Nachweisgrenze 15.6 pg/mL
Applikation ELISA
Pubmed 1 Publikation vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails TNFSF12 ELISA Kit

Antigendetails Anwendungsinformationen Handhabung ProductDetails: References for TNFSF12 Kit (ABIN1889351) Bilder
Verwendungszweck Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse TNFSF12/TWEAK
Marke PicoKine™
Proben Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Nachweismethode Colorimetric
Analytische Methode Quantitative
Spezifität Expression system for standard: E.coli
Immunogen sequence: S94-H249
Kreuzreaktivität (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivität <10pg/mL
Benötigtes Material Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: E.coli
Immunogen sequence: S94-H249

Antigendetails

Produktdetails TNFSF12 ELISA Kit Anwendungsinformationen Handhabung ProductDetails: References for TNFSF12 Kit (ABIN1889351) Bilder zurück nach oben
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Andere Bezeichnung TNFSF12 (TNFSF12 ELISA Kit Abstract)
Hintergrund

Protein Function: Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines. Promotes IL8 secretion (By similarity). .

Background: Tumor necrosis factor ligand superfamily member 12 also known as TNF-related weak inducer of apoptosis(TWEAK) is a protein that in humans is encoded by the TNFSF12 gene. The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor(TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. This cytokine has overlapping signaling functions with TNF, but displays a much wider tissue distribution. This cytokine can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator ofangiogenesis. The TNFSF12 gene lies 878 bp upstream of the putative transcriptional start site of the TNFSF13 gene on chromosome 17p13.1.

Synonyms: Tumor necrosis factor ligand superfamily member 12,TNF-related weak inducer of apoptosis,TWEAK,Tumor necrosis factor ligand superfamily member 12, membrane form,Tumor necrosis factor ligand superfamily member 12, secreted form,Tnfsf12,

Full Gene Name: Tumor necrosis factor ligand superfamily member 12

Cellular Localisation: Cell membrane, Single-pass type II membrane protein.
Gen-ID 21944
UniProt O54907
Pathways Apoptose

Anwendungsinformationen

Produktdetails TNFSF12 ELISA Kit Antigendetails Handhabung ProductDetails: References for TNFSF12 Kit (ABIN1889351) Bilder zurück nach oben
Applikationshinweise Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Kommentare

Tissue Specificity: Widely expressed.

Plattentyp Pre-coated
Protokoll mouse TNFSF12 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TNFSF12 has been precoated onto 96-well plates. Standards(E.coli, S94-H249) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFSF12 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TNFSF12 amount of sample captured in plate.
Testdurchführung Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse TNFSF12 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well.. See "Sample Dilution Guideline" above for details. It is recommended that each mouse TNFSF12 standard solution and each sample be measured in duplicate.
Testpräzision
  • Sample 1: n=16, Mean(pg/ml): 84, Standard deviation: 3.53, CV(%): 4.2
  • Sample 2: n=16, Mean(pg/ml): 210, Standard deviation: 7.35, CV(%): 3.5
  • Sample 3: n=16, Mean(pg/ml): 537, Standard deviation: 27.4, CV(%): 5.1,
  • Sample 1: n=24, Mean(pg/ml): 106, Standard deviation: 5.62, CV(%): 5.3
  • Sample 2: n=24, Mean(pg/ml): 324, Standard deviation: 14.91, CV(%): 4.6
  • Sample 3: n=24, Mean(pg/ml): 638, Standard deviation: 38.28, CV(%): 6
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Handhabung

Produktdetails TNFSF12 ELISA Kit Antigendetails Anwendungsinformationen ProductDetails: References for TNFSF12 Kit (ABIN1889351) Bilder zurück nach oben
Handhabung Avoid multiple freeze-thaw cycles.
Lagerung -20 °C,4 °C
Informationen zur Lagerung Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Haltbarkeit 12 months

ProductDetails: References for TNFSF12 Kit (ABIN1889351)

Produktdetails TNFSF12 ELISA Kit Antigendetails Anwendungsinformationen Handhabung Bilder zurück nach oben
Allgemeine Veröffentlichungen

Chicheportiche, Bourdon, Xu, Hsu, Scott, Hession, Garcia, Browning: "TWEAK, a new secreted ligand in the tumor necrosis factor family that weakly induces apoptosis." in: The Journal of biological chemistry, Vol. 272, Issue 51, pp. 32401-10, 1998

Bilder

Produktdetails TNFSF12 ELISA Kit Antigendetails Anwendungsinformationen Handhabung ProductDetails: References for TNFSF12 Kit (ABIN1889351) zurück nach oben
Bilder des Herstellers
ELISA image for TNFSF12 ELISA Kit (Tumor Necrosis Factor (Ligand) Superfamily, Member 12) (ABIN1889351) Mouse TNFSF12/TWEAK PicoKine ELISA Kit standard curve