anti-Ratte (Rattus) Caspase 8 Antikörper für Western Blotting

Recommended Caspase 8 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) Antikörper
  • casp8-A
  • xCaspase-8
  • casp8
  • CASP8
  • CASP-8
  • FLICE
  • MACH
  • Mch5
  • ALPS2B
  • CAP4
  • Casp-8
  • MCH5
  • caspase-8
  • zgc:92075
  • caspase 8, apoptosis-related cysteine peptidase
  • death related ced-3/Nedd2-like protein
  • caspase-8
  • caspase 8
  • casp8
  • Dredd
  • CASP8
  • AaeL_AAEL014148
  • LOC100222284
  • Casp8
  • LOC100348477
Epitop
AA 430-482
43
19
18
16
14
12
10
10
9
8
7
6
6
6
6
5
5
5
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
360
120
95
25
21
20
3
2
1
Wirt
Kaninchen
278
115
10
2
Klonalität
Polyklonal
Konjugat
Dieser Caspase 8 Antikörper ist unkonjugiert
18
13
8
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Applikation
Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
360
109
92
78
51
36
36
28
13
12
9
8
6
5
2
2
2
1
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen

Bilder

Produktnummer ABIN724205
$ 307.45
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
3.0762544 ABIN222878 WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 5
3.0762544 ABIN223020 WB Rabbit IgG active Anmelden zum Anzeigen Polyclonal 4
3.0762544 ABIN1532233 IHC ELISA WB Rabbit IgG AA 313-362 Anmelden zum Anzeigen Polyclonal
3.0762544 ABIN1531215 IHC ELISA WB Rabbit IgG AA 313-362, pSer347 Anmelden zum Anzeigen Polyclonal
3.0762544 ABIN4288172 ICC IF IHC IHC (p) WB Rabbit IgG Center Anmelden zum Anzeigen Polyclonal 1
3.0762544 ABIN252118 ICC IHC (fro) IF IHC (p) IP IHC WB Rabbit Large Subunit, Small Subunit, active Anmelden zum Anzeigen Polyclonal 33
3.0762544 ABIN969003 FACS IHC ELISA WB Mouse IgG1 Anmelden zum Anzeigen 1H11 2
3.0762544 ABIN1105677 WB Rabbit IgG Middle Region Anmelden zum Anzeigen Polyclonal
3.0762544 ABIN3044361 WB Rabbit IgG AA 240-259, Middle Region Anmelden zum Anzeigen Polyclonal 9
3.0762544 ABIN2477922 WB Rabbit IgG C-Term Anmelden zum Anzeigen Polyclonal 3
3.0762544 ABIN3183671 ELISA IHC WB Rabbit IgG Ser2 Anmelden zum Anzeigen Polyclonal
3.0762544 ABIN3181527 IHC WB Mouse IgG1 Anmelden zum Anzeigen 2G12
3.0762544 ABIN2705693 WB Rabbit pTyr380 Anmelden zum Anzeigen Polyclonal
3.0762544 ABIN2705694 IHC WB Rabbit Center Anmelden zum Anzeigen Polyclonal
3.0762544 ABIN3188046 IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal
3.0762544 ABIN4288165 ELISA ICC IF IHC IHC (p) WB Rabbit C-Term Anmelden zum Anzeigen Polyclonal
3.0762544 ABIN4288173 ELISA FACS IHC IHC (p) WB Mouse IgG1 Anmelden zum Anzeigen 1H11
3.0762544 ABIN2852721 IHC WB Rabbit Anmelden zum Anzeigen Polyclonal
3.0762544 ABIN2852720 IHC WB Mouse Anmelden zum Anzeigen
3.0762544 ABIN1105678 EIA FACS IHC (p) WB Mouse IgG1 Anmelden zum Anzeigen 1H11

General

Antigen Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) Antikörper
Epitop AA 430-482
(43), (19), (18), (16), (14), (12), (10), (10), (9), (8), (7), (6), (6), (6), (6), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human, Maus, Ratte (Rattus)
(360), (120), (95), (25), (21), (20), (3), (2), (1)
Wirt Kaninchen
(278), (115), (10), (2)
Klonalität
Polyklonal
Konjugat Dieser Caspase 8 Antikörper ist unkonjugiert
(18), (13), (8), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(360), (109), (92), (78), (51), (36), (36), (28), (13), (12), (9), (8), (6), (5), (2), (2), (2), (1), (1), (1), (1)
Pubmed 10 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-Caspase 8 Antikörper

Target Details Caspase 8 Anwendungsinformationen Handhabung Referenzen für anti-Caspase 8 Antikörper (ABIN724205) Bilder
Reinigung Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from mouse Caspase-8 subunit p10
Isotyp IgG

Target Details Caspase 8

Produktdetails anti-Caspase 8 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-Caspase 8 Antikörper (ABIN724205) Bilder zurück nach oben
Antigen
Andere Bezeichnung Caspase 8 (CASP8 Antibody Abstract)
Hintergrund

Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP1. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein.

Subcellular location: Cytoplasm

Synonyms: MACH, Mch5, FLICE, CASP-8, Caspase-8, Casp8

Gen-ID 12370
UniProt O89110
Forschungsgebiet Proteolysis / Ubiquitin, Apoptosis/Necrosis
Pathways Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades

Anwendungsinformationen

Produktdetails anti-Caspase 8 Antikörper Target Details Caspase 8 Handhabung Referenzen für anti-Caspase 8 Antikörper (ABIN724205) Bilder zurück nach oben
Applikationshinweise WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-Caspase 8 Antikörper Target Details Caspase 8 Anwendungsinformationen Referenzen für anti-Caspase 8 Antikörper (ABIN724205) Bilder zurück nach oben
Format Liquid
Konzentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20°C
Haltbarkeit 12 months

Referenzen für anti-Caspase 8 Antikörper (ABIN724205)

Produktdetails anti-Caspase 8 Antikörper Target Details Caspase 8 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Tulsulkar, Glueck, Hinds, Shah: "Ginkgo biloba Extract Prevents Female Mice from Ischemic Brain Damage and the Mechanism Is Independent of the HO1/Wnt Pathway." in: Translational stroke research, Vol. 7, Issue 2, pp. 120-31, 2016

Akinrinde, Oyagbemi, Omobowale, Asenuga, Ajibade: "Alterations in blood pressure, antioxidant status and caspase 8 expression in cobalt chloride-induced cardio-renal dysfunction are reversed by Ocimum gratissimum and gallic acid in Wistar rats." in: Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS), Vol. 36, pp. 27-37, 2016 (Probematerial (Species): Rat (Rattus)).

Gao, Liu, Li, Wu, Peng, Jing: "Hispidulin induces mitochondrial apoptosis in acute myeloid leukemia cells by targeting extracellular matrix metalloproteinase inducer." in: American journal of translational research, Vol. 8, Issue 2, pp. 1115-32, 2016

Daverey, Agrawal: "Curcumin alleviates oxidative stress and mitochondrial dysfunction in astrocytes." in: Neuroscience, Vol. 333, pp. 92-103, 2016

Wang, Zhao, Yu, Feng, Zhang, Kou, Chu, Cui, Li, Zhang, Shen, Min: "Regulation of steroid hormones and energy status with cysteamine and its effect on spermatogenesis." in: Toxicology and applied pharmacology, Vol. 313, pp. 149-158, 2016

Zhao, Zhang, Liu, Zhang, Hao, Li, Chen, Shen, Tang, Min, Meng, Wang, Yi, Zhang: "Hydrogen Sulfide and/or Ammonia Reduces Spermatozoa Motility through AMPK/AKT Related Pathways." in: Scientific reports, Vol. 6, pp. 37884, 2016 Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Pig (Porcine)).

Fang, Zhang, Qi, Fan, Luo, Liu, Tan: "Evodiamine induces G2/M arrest and apoptosis via mitochondrial and endoplasmic reticulum pathways in H446 and H1688 human small-cell lung cancer cells." in: PLoS ONE, Vol. 9, Issue 12, pp. e115204, 2014

Wang, Han, Zhou, Wu, Wang, Jia, Lv, Cheng, Pan, Liu, Zhou, Sun: "Inhibition of hydrogen sulfide synthesis provides protection for severe acute pancreatitis rats via apoptosis pathway." in: Apoptosis : an international journal on programmed cell death, Vol. 18, Issue 1, pp. 28-42, 2013

Wu, Tang, Jiang, Li, Jiang, Liu: "PCSK9 siRNA inhibits HUVEC apoptosis induced by ox-LDL via Bcl/Bax-caspase9-caspase3 pathway." in: Molecular and cellular biochemistry, Vol. 359, Issue 1-2, pp. 347-58, 2011

Jeng, Dyson: "Direct measurement of the aspartic acid 26 pKa for reduced Escherichia coli thioredoxin by 13C NMR." in: Biochemistry, Vol. 35, Issue 1, pp. 1-6, 1996

Bilder

Produktdetails anti-Caspase 8 Antikörper Target Details Caspase 8 Anwendungsinformationen Handhabung Referenzen für anti-Caspase 8 Antikörper (ABIN724205) zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) (AA 430-482) antibody (ABIN724205) Formalin-fixed and paraffin embedded human rectal carcinoma labeled with Anti-Caspase...
SDS-PAGE (SDS) image for anti-Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) (AA 430-482) antibody (ABIN724205) L1 mouse liver lysates L2 mouse spleen lysates probed with Anti Caspase 8 Polyclonal ...
Western Blotting (WB) image for anti-Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) (AA 430-482) antibody (ABIN724205) Image provided by the Independent Validation Program (badge number 29759). Lane 1: He...
Immunofluorescence (Cultured Cells) (IF (cc)) image for anti-Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) (AA 430-482) antibody (ABIN724205) Image provided by One World Lab validation program. MCF-7 cells probed with Rabbit An...
Immunofluorescence (Cultured Cells) (IF (cc)) image for anti-Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) (AA 430-482) antibody (ABIN724205) Image provided by One World Lab validation program. A431 cells probed with Rabbit Ant...
'Independent Validation' Siegel
Chargennummer 131127
Validierte Anwendung Western Blotting
Positivkontrolle HeLa cells
Negativkontrolle c6/36 mosquito cells (non-reactive species)
Bewertung A strong band was observed at the correct molecular weight in the positive control sample. No major bands were observed in the negative sample.
Primärantikörper
Sekundärantikörper
Kontrollen
  • Positive control: HeLa cell extract
  • Negative control: c6/36 cell extract
Protokoll
  • 1. Total protein extracts were boiled in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol at 95°C for 5 min prior to loading.
  • 2. 20 μg of boiled extracts were loaded and resolved on 8-16% SDS-polyacrylamide gel.
  • 3. The Thermo Scientific - Spectra Multicolor Broad Range (Cat # 26634) were used as molecular mass markers.
  • 4. Proteins were then transferred onto PVDF membrane by wet transfer and protein transfer was confirmed with Ponceau-S staining.
  • 5. The PVDF membrane was incubated with 25 mL of blocking buffer [Tris Buffered Saline, pH 7.4 plus 0.1% TW20 (TBST)] containing 5% (W/V) BSA at room temperature for 1 h.
  • 6. The membrane was rinsed with TBST once.
  • 7. The membrane was immersed with the protein side up in the primary antibody solution (CASP8; 1:200) in TBST containing 5% (W/V) BSA and incubated for 16 h at 4°C.
  • 8. The membrane was rinsed in TBST thrice for 5 min each.
  • 9. The membrane was incubated in the HRP-conjugated secondary antibody solution (Goat anti-rabbit IgG-HRP; 1:20,000) in TBST containing 5% (W/V) BSA and incubated for 1 hour at room temperature (~26°C) with gentle agitation.
  • 10. The membrane was rinsed thrice TBST thrice for 5 min each.
  • 11. The membrane was rinsed in TBS twice for 30 s each.
  • 12. Signals were detected with ECL-2 Substrate. The blot was scanned for 300 s.
  • 13. The membrane was rinsed three times TBST.
  • 14. Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 min each.
  • 15. The membrane was washed in TBST 2 times for 10 min each.
  • 16. Repeated Steps 5-12 with the loading control antibody (anti-Actin; 1:6,000) and its matching secondary antibody (Goat anti-rabbit IgG-HRP; 1:20,000).
Anmerkungen - Nothing to note.
Validierungsbilder
Western Blotting validation image for anti-Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) (AA 430-482) antibody (ABIN724205) Figure 1: Western blot of lysates from HeLa cells (Lane 1), and c6/36 cells (Lane 2) ...